Method for producing sweet juice from lignocellulosic biomass with improved enzyme recycle
AssigneeIfp
InventorsDominique Casanave, Nicolas Lopes Ferreira
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Abstract
The present invention relates to a method of producing sweet juice from lignocellulosic substrate, wherein enzymatic hydrolysis is carried out by performing cellulase supplementation with supported β-glucosidases used in a reactor separate from the lignocellulose enzymatic hydrolysis reactor.
Claims20
A method of producing sweet juice from lignocellulosic substrate by enzymatic hydrolysis, wherein cellulase supplementation is carried out with supported β-glucosidases, in a reactor (2) separate from a lignocellulose enzymatic hydrolysis reactor (1), wherein said lignocellulosic substrate, which has been pretreated beforehand to improve digestibility, is contacted with a cellulase solution, hydrolysate H1 obtained at the outlet of reactor (1) being sent to a separation means for extracting the cellulases prior to being sent to reactor (2), said method comprising a lignocellulose conversion stage, a sweet juice production stage and a stage of complete emptying of the reactors.
A device intended for sweet juice production by enzymatic hydrolysis from lignocellulosic substrate comprising:
- at least a first lignocellulose enzymatic hydrolysis reactor (1), said reactor (1) being provided with at least one line for introducing the substrate and the cellulase solution, at least one line for withdrawing the converted juice (H1) and sending it to at least one filter medium (3) arranged at the outlet of reactor (1), and at least one separation means (7), for extracting the cellulases from the hydrolysate,
- optionally a heat exchanger at the outlet of the first reactor,
- at least a second reactor (2) comprising the supported β-glucosidases, connected to at least one separation means (7) by at least one line, said reactor (2) being equipped at the outlet thereof with at least one filter medium (4) and at least one line for extracting stream (H2) comprising the hydrolysate poor in soluble oligomers,
- at least one line for feeding at least part of stream (H2) into a contactor (5) and at least one line for sending back at least part of stream (H2) to reactor (1),
- at least one contactor (5) provided with at least one line for introducing the new substrate, at least one line for extracting the sweet juice produced (H3) and at least one line for discharging the cellulase-impregnated new substrate and feeding it into reactor (1).
Dependencies are inferred from the claim text.