Method for manufacturing a tissue-engineered construct
AssigneesTechnische Universiteit Eindhoven, Stichting voor de Technische Wetenschappen
InventorsJeroen Kortsmit, Niels Jozef Bart Driessen, Marcel Cornelis Maria Rutten, Franciscus Petrus Thomas Baaijens
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Abstract
Method for manufacturing a tissue-engineered construct, such as a heart valve, comprising the steps of providing a-cell-seeded scaffold in a bioreactor chamber which bioreactor chamber is divided by the cell-seeded scaffold into a first compartment and a second compartment, subjecting the cell-seeded scaffold to a flow of nutrient medium within the bioreactor chamber for developing the cell-seeded scaffold to a tissue structure and next to the tissue construct, applying a dynamic pressure difference to the developing tissue structure by the flow of nutrient medium to induce dynamic strain on the tissue structure.
Claims17
1-16. (canceled)
Method for manufacturing a tissue-engineered construct, such as a heart valve, comprising the steps of
- providing a cell-seeded scaffold in a bioreactor chamber which bioreactor chamber is divided by the cell-seeded scaffold into a first compartment and a second compartment,
- subjecting the cell-seeded scaffold to a flow of nutrient medium within the bioreactor chamber for developing the cell-seeded scaffold to a tissue structure and next to the tissue construct,
- applying a dynamic pressure difference to the developing tissue structure by the flow of nutrient medium to induce dynamic strain on the tissue structure
- measuring the dynamic pressure difference,
- measuring the volumetric deformation of the developing tissue structure,
- determining the magnitude of at least one mechanical property of the tissue structure from the measured volumetric deformation and from applied pressure difference over the tissue structure while the tissue structure is within the bioreactor chamber.
- determining if the determined magnitude of at least one mechanical property exceeds a certain threshold value
- continuing the subjection of the tissue structure to a flow of nutrient medium if the determined magnitude of at least one mechanical property does not exceed the threshold value,
- ending the subjection of the tissue structure to a flow of nutrient medium if the determined magnitude of at least one mechanical property exceeds the threshold value.
Method for testing a tissue construct, such as a heart valve, comprising the steps of
- providing a tissue construct to be tested in a bioreactor chamber which bioreactor chamber is divided by the tissue construct into a first compartment and a second compartment,
- applying a dynamic pressure difference to the tissue structure to induce dynamic strain on the tissue structure,
- measuring the dynamic pressure difference,
- measuring the volumetric deformation of the tissue construct,
- determining the magnitude of at least one mechanical property of the tissue construct from the measured volumetric deformation and from the applied pressure difference over the tissue structure.
Dependencies are inferred from the claim text.