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US 2011/0033880 A1Published application
Fad-conjugated glucose dehydrogenase gene
AssigneeIkeda Food Research Co., Ltd.
InventorsTakako Yada, Koji Miyamoto, Michinari Honda
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AssigneeIkeda Food Research Co., Ltd.
InventorsTakako Yada, Koji Miyamoto, Michinari Honda
Estimating the term…
An object of the present invention is to provide: a novel gene (polynucleotide) encoding an FAD-conjugated glucose dehydrogenase having excellent properties that it has excellent reactivity to glucose, excellent thermal stability, and excellent substrate-recognition performance and also has a low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; and a method for the determination of glucose, a reagent composition for use in the determination of glucose, a biosensor for use in the determination of glucose and others, each characterized by using the enzyme obtained. The invention relates to a polynucleotide encoding an FAD-conjugated glucose dehydrogenase, comprising a polypeptide containing an amino acid sequence: X1-X2-X3-X4-X5-X6 (wherein X1 and X2 independently represent an aliphatic amino acid residue; X3 and X6 independently represent a branched amino acid residue; and X4 and X5 independently represent a heterocyclic amino acid residue or an aromatic amino acid residue); and others.
A polynucleotide encoding an FAD-conjugated glucose dehydrogenase, comprising a polypeptide containing an amino acid sequence:
A polynucleotide encoding a polypeptide (a), (b) or (c) defined below:
A polynucleotide (d), (e) or (f) defined below:
A polynucleotide which has a DNA fragment amplifiable by PCR using a combination of a sense primer comprising a base sequence encoding the amino acid sequence:
A polynucleotide which hybridizes to a probe comprising a base sequence encoding the amino acid sequence:
A polynucleotide encoding an FAD-conjugated glucose dehydrogenase derived from Aspergillus oryzae, characterized by showing a value of enzymatic activity for maltose of 10% or less and a value of enzymatic activity for D-galactose of 5% or less with a value of enzymatic activity for D-glucose taken as 100%.
A polynucleotide encoding an FAD-conjugated glucose dehydrogenase derived from Aspergillus oryzae, characterized by having an enzymatic activity of 300 U/mg or more.
Dependencies are inferred from the claim text.