Direct assay of cholesterol in skin removed by tape stripping
AssigneeMiraculins Inc.
InventorsPeter Horsewood, Robert Zawydiwski
Life of this patent
Estimating the term…
Abstract
Skin cholesterol is measured by applying an adhesive tape onto a selected area of the skin to adhere the tape to the selected skin area and stripping the tape off the selected skin area to obtain a sample representative of the outer stratum corneum layer of the skin, the sample adhering to the tape so as to have exposed skin constituents. The sample is assayed using a detector reagent that specifically binds to cholesterol and in addition has an indicator component that allows quantitation of the amount of cholesterol present in the exposed skin constituents.
Claims26
1-58. (canceled)
A tape for measuring skin cholesterol comprising a backing member coated on at least one side thereof with a medical adhesive for applying the tape onto a selected area of skin to adhere the tape to the selected skin area, said tape suitable for stripping the tape off the selected skin area to obtain a sample representative of an outer stratum corneum layer of the skin, the sample adhering to the tape so as to have exposed skin constituents, and suitable for applying a source of an affinity signal-generating compound of formula A-C-B′, wherein A is a detecting agent having affinity for cholesterol, B′ is a signal-generating indicator agent and C is a binding agent linking said detecting agent and said indicator agent to one another, onto a predetermined surface area of said sample, to provide a value indicative of cholesterol level.
(canceled)
(canceled)
64-89. (canceled)
A tape for measuring skin cholesterol, comprising a backing member coated on at least one side thereof with a medical adhesive, suitable for applying the tape onto a selected area of skin to adhere the tape to the selected skin area, for stripping the tape off the selected skin area to obtain a sample representative of an outer stratum corneum layer of the skin, the sample adhering to the tape so as to have exposed skin constituents, said tape suitable for applying a source of cholesterol oxidase as a detecting agent having affinity for cholesterol, for measuring the amount of hydrogen peroxide formed being indicative of cholesterol level.
94-109. (canceled)
A tape stripping device for use in obtaining skin samples, the device comprising:
- a) a sampling member including an interior surface and an outer surface, either the interior surface or the outer surface including at least one indentation delineating at least one releasable section;
- b) a tape provided on the interior surface of the sampling member, the tape covering at least a portion of the at least one releasable section, the tape having a medical adhesive presented away from the interior surface;
- and
- c) a closure member adapted to engage the sampling member and seal the tape within the device.
(canceled)
(canceled)
Dependencies are inferred from the claim text.