Enzyme Triggered Redox Altering Chemical Elimination (E-Trace) Immunoassay
AssigneeOHMX Corporation
InventorPaul A. Bertin
Life of this patent
Estimating the term…
Abstract
The present invention is directed to electronic methods of detecting target analytes such that upon binding of the target analyte a shift in electrochemical potential is seen. This assay relies on the use of an electroactive moiety (“EAM”) that is attached to an electrode and comprises a self-immolative moiety, whose presence gives the EAM a first E0, and whose absence, upon irreversible cleavage gives the EAM a second E0. This difference is detected, and if such change occurs, it is an indication of the presence of a target analyte.
Claims9
A method for detecting a target analyte in a test sample, said method comprising:
- (a) providing a solid support comprising an electrode comprising:
- (i) a self-assembled monolayer (SAM);
- (ii) a covalently attached electroactive active moiety (EAM) comprising a transition metal complex comprising a self-immolative moiety and a peroxide sensitive moiety (PSM), wherein said EAM has a first E0,
- (iii) a capture binding ligand that binds said analyte;
- (b) contacting said target analyte and said solid support, under conditions wherein said target analyte binds said capture binding ligand to form a first complex;
- (c) contacting said first complex with a soluble capture ligand that binds said target analyte, wherein said soluble capture ligand comprises a peroxide generating moiety to form a second complex;
- (d) adding a peroxide substrate to said second complex under conditions that peroxide is generated and said self-immolative moiety is removed such that said EAM has a second E0;
- and
- (e) detecting said second E0 as an indication of the presence of said target.
A composition comprising a solid support comprising:
- (a) an electrode comprising:
- (i) a self-assembled monolayer (SAM);
- (ii) a covalently attached electroactive active moiety (EAM) comprising a transition metal complex comprising a self-immolative moiety and a peroxide sensitive moiety (PSM), wherein said EAM has a first E0 when said self-immolative moiety is covalently attached to said EAM and a second E0 when said self-immolative moiety is absent;
- (iii) a capture binding ligand that binds said analyte;
- and
- (b) a soluble capture ligand that binds said target analyte, wherein said soluble capture ligand comprises a peroxide generating moiety.
Dependencies are inferred from the claim text.