Rna-selective hybridization reagent and use of the same
AssigneeJapan Science and Technology Agency
InventorsYoshihito Ueno, Yukio Kitade
Life of this patent
Estimating the term…
Abstract
Provided is a nucleoside derivative which has a high affinity for RNA. Use is made of a nucleoside derivative represented by either formula (1) or formula (2). (In formulae (1) and (2), Z represents a carbon atom or a nitrogen atom; R1 represents a hydrogen atom or a hydroxyl-protecting group; and R2 represents a hydrogen atom or a phosphodiester group).
Claims11
1-4. (canceled)
An RNA hybridization reagent provided with one species or two or more species of nucleotide derivative units represented by any of the following Formulae (5) and (6):
- (Where Z represents a carbon atom or a nitrogen atom;
- X1 represents 0, S or Se;
- X2 represents SH (or S−), S or Se−, or an alkyl group having 1 to 4 carbons or a morpholino group.)
A probe set for detecting a mutation on an RNA, comprising:
- a first probe provided with one species or two or more species of nucleotide derivative units represented by any of the following Formulae (5) and (6) at the 5′ end or the 3′ end corresponding to a site of the mutation;
- and
- one species or two or more species of second probes provided with a deoxynucleotide having a base complementary to a base that has the possibility to be present in a site of the mutation at the 3′ end or the 5′ end corresponding to the site of the mutation:
- (Where Z represents a carbon atom or a nitrogen atom;
- X1 represents 0, S or Se;
- X2 represents SH (or S−), S or Se−, or an alkyl group having 1 to 4 carbons or a morpholino group.)
A detecting method for a single base polymorphism, comprising the steps of:
- preparing an RNA sample as a gene expression product having the possibility of containing the single base polymorphism;
- with a probe set being provided containing a first probe provided with a nucleotide derivative unit represented by any of the following Formulae (5) and (6) at the 5′ end or the 3′ end corresponding to a site of the single base polymorphism, and one species or two or more species of second probes provided with a deoxynucleotide having a base complementary to a base that has the possibility to be present in a site of the single base polymorphism at the 3′ end or the 5′ end corresponding to the site of the single base polymorphism,
- causing the first probe, the second probe and the RNA sample to contact one another allowing hybridization in combinations of one species or two or more species obtained by combining one species of the first probe and one species of the second probe selected from the probe set,
- (Where Z represents a carbon atom or a nitrogen atom;
- X1 represents 0, S or Se;
- X2 represents SH (or S−), S or Se−, or an alkyl group having 1 to 4 carbons or a morpholine group.);
- and
- detecting a fluorescence signal based on the first probe which is a hybridization product among the RNA sample, the first probe and the second probe.
Dependencies are inferred from the claim text.