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US 2011/0033845 A1Published application
Methods For Amplification of Nucleic Acids Using Spanning Primers
AssigneeLIFE TECHNOLOGIES CORPORATION, a Delaware Corporation
InventorEugene G. SPIER
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Abstract
The teachings relate to methods and kits for detecting whether target nucleic acid sequences are present and/or quantitating target nucleic acid sequences.
Claims10
A method for amplifying at least one target nucleic acid sequence comprising:
- forming an amplification reaction composition comprising:
- a target nucleic acid sequence;
- a polymerase;
- a first primer comprising (i) a sequence complementary to the 5′ end of the target nucleic acid sequence and (ii) a sequence complementary to the 3′ end of the target nucleic acid sequence;
- and
- subjecting the amplification reaction composition to at least one amplification reaction to form at least one amplification product.
3 more elements
Dependencies are inferred from the claim text.