Methods of Detecting Signatures of Disease or Conditions in Bodily Fluids
AssigneePresident and Fellows of Harvard College
InventorAmin I. Kassis
Life of this patent
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Abstract
Methods and compositions for diagnosing the presence of a cancer cell in an individual are provided. Methods and compositions for identifying a tumor-specific signature in an individual having cancer are also provided. Methods and compositions for diagnosing the presence of an infectious agent in an individual and/or for identifying an infectious agent-specific signature in an infected individual are provided. Methods and compositions for diagnosing the presence of a disease in an individual are also provided. Methods and compositions for identifying a disease-specific signature in an individual having the disease are also provided.
Claims60
A method for diagnosing the presence of a cancer cell in an individual comprising the steps of:
- obtaining a first expression profile from a blood phagocytic cell from an individual;
- obtaining a second expression profile from a blood non-phagocytic cell from the individual;
- comparing the first and second expression profiles;
- identifying differential expression of one or more markers specific to the first expression profile;
- and
- relating the differential expression of the one or more markers specific to the first expression profile to the presence of a cancer cell in the individual.
A method for identifying a tumor-specific signature in an individual having cancer comprising the steps of:
- obtaining a first expression profile from a blood phagocytic cell from an individual having cancer;
- obtaining a second expression profile from a blood non-phagocytic cell from the individual having cancer;
- comparing the first and second expression profiles;
- identifying differential expression of two or more markers specific to the first expression profile;
- and
- relating the differential expression of the two or more markers specific to a tumor-specific signature in the individual having cancer.
A method for diagnosing the presence of a cancer cell in an individual comprising the steps of:
- obtaining a first expression profile from a blood phagocytic cell from an individual;
- obtaining a second expression profile from a blood non-phagocytic cell from the individual;
- comparing the first and second expression profiles;
- identifying the presence of a circulating tumor cell or subcellular fragment thereof specific to the first expression profile;
- and
- relating the presence of a circulating tumor cell or subcellular fragment thereof to the presence of a cancer cell in the individual.
A method for diagnosing the presence of a cancer cell in an individual comprising the steps of:
- isolating a population of phagocytic cells from an individual;
- separating 2n phagocytic cells from >2n phagocytic cells;
- obtaining a first expression profile from the 2n phagocytic cells;
- obtaining a second expression profile from the >2n phagocytic cells;
- comparing the first and second expression profiles;
- identifying differential expression of one or more markers specific to the first expression profile;
- and
- relating the differential expression of the one or more markers specific to the first expression profile to the presence of a cancer cell in the individual.
A method for diagnosing the presence of an infectious agent in an individual comprising the steps of:
- obtaining a first expression profile from a blood phagocytic cell from an individual;
- obtaining a second expression profile from a blood non-phagocytic cell from the individual;
- comparing the first and second expression profiles;
- identifying differential expression of one or more markers specific to the first expression profile;
- and
- relating the differential expression of the one or more markers specific to the first expression profile to the presence of an infectious agent in the individual.
A method for identifying an infectious agent-specific signature in an infected individual comprising the steps of:
- obtaining a first expression profile from a blood phagocytic cell from an infected individual;
- obtaining a second expression profile from a blood non-phagocytic cell from the infected individual;
- comparing the first and second expression profiles;
- identifying differential expression of two or more markers specific to the first expression profile;
- and
- relating the differential expression of the two or more markers specific to an infectious agent-specific signature in the infected individual.
A method for diagnosing the presence of an infectious agent in an individual comprising the steps of:
- obtaining a first expression profile from a blood phagocytic cell from an individual;
- obtaining a second expression profile from a blood non-phagocytic cell from the individual;
- comparing the first and second expression profiles;
- identifying the presence of a circulating tumor cell or subcellular fragment thereof specific to the first expression profile;
- and
- relating the presence of a circulating tumor cell or subcellular fragment thereof to the presence of an infectious agent in the individual.
A method for diagnosing the presence of an infectious agent in an individual comprising the steps of:
- isolating a population of phagocytic cells from an individual;
- separating 2n phagocytic cells from >2n phagocytic cells;
- obtaining a first expression profile from the 2n phagocytic cells;
- obtaining a second expression profile from the >2n phagocytic cells;
- comparing the first and second expression profiles;
- identifying differential expression of one or more markers specific to the first expression profile;
- and
- relating the differential expression of the one or more markers specific to the first expression profile to the presence of an infectious agent in the individual.
One or more markers of a condition differentially expressed between phagocytic cells and nonphagocytic cells from a common source.
One or more markers of a condition differentially expressed between phagocytic cells having a DNA content of 2n and phagocytic cells having a DNA content of greater than 2n from a common source.
Protein profile, DNA profile, RNA profile, metabolite profile, glycome profile, glycoprotein profile, carbohydrate profile, lipoprotein profile or lipid profile specific to blood phagocytic cells compared to blood nonphagocytic cells from a common source.
Protein profile, DNA profile, RNA profile, metabolite profile, glycome profile, glycoprotein profile, carbohydrate profile, lipoprotein profile or lipid profile specific to blood phagocytic cells having a DNA content of greater than 2n compared to blood phagocytic cells having a DNA content of 2n from a common source.
A kit for detecting the presence of one or more markers associated with cancer and/or an infectious agent in a biological sample, wherein the kit comprises a labeled compound or agent for detecting a marker polypeptide, protein lipid, oligosaccharide, mRNA, microRNA, or genomic DNA, and a standard such as a non-phagocytic cell or a 2n cell to compare the amount of marker in the sample with the standard
Dependencies are inferred from the claim text.