Anti-Glypican 3 Antibody Having Modified Sugar Chain

A composition comprising an anti-glypican 3 antibody, wherein the anti-glypican 3 antibody comprises a light chain variable region comprising a CDR1 comprising the amino acid sequence of SEQ ID NO:45, a CDR2 comprising the amino acid sequence of SEQ ID NO:46, and a CDR3 comprising the amino acid sequence of SEQ ID NO:60, wherein the sugar chain component of the antibody has been modified.

A composition comprising an anti-glypican 3 antibody, wherein the anti-glypican 3 antibody comprises a heavy chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID NO:81, SEQ ID NO:82, SEQ ID NO:83, SEQ ID NO:84, SEQ ID NO:85, SEQ ID NO:86, and SEQ ID NO:87, and wherein the sugar chain component of the antibody has been modified.

A composition comprising an anti-glypican 3 antibody, wherein the anti-glypican 3 antibody comprises a light chain variable region comprising the amino acid sequence of SEQ ID NO:109, and wherein the sugar chain component of the antibody has been modified.

A composition comprising an anti-glypican 3 antibody, wherein the anti-glypican 3 antibody comprises a light chain variable region comprising a CDR2 comprising the amino acid sequence of SEQ ID NO:46, a CDR3 comprising the amino acid sequence of SEQ ID NO:60, and a CDR1 comprising an amino acid sequence selected from the group consisting of SEQ ID NO:90, SEQ ID NO:91, SEQ ID NO:92, SEQ ID NO:93, SEQ ID NO:94, SEQ ID NO:95, SEQ ID NO:96, SEQ ID NO:97, SEQ ID NO:98, SEQ ID NO:99, SEQ ID NO:100, SEQ ID NO:101, SEQ ID NO:102, SEQ ID NO:103, and SEQ ID NO:104.

A method for producing an anti-glypican 3 antibody with a modified sugar chain component, the method comprising:

• (a) providing a cell expressing a nucleic acid encoding an anti-glypican 3 antibody, wherein the cell is a mutant cell that has a reduced capability of adding fucose to sugar chains as compared to a corresponding wild type cell;
• and
• (b) culturing the mutant cell, thereby producing the anti-glypican 3 antibody with a modified sugar chain component, wherein the anti-glypican 3 antibody comprises a heavy chain variable region comprising a CDR1 comprising the amino acid sequence of SEQ ID NO:25, a CDR2 comprising the amino acid sequence of SEQ ID NO:26, and a CDR3 comprising the amino acid sequence of SEQ ID NO:27.

A method for producing an anti-glypican 3 antibody with a modified sugar chain component, the method comprising:

• (a) providing a cell expressing a nucleic acid encoding an anti-glypican 3 antibody, wherein the cell is a mutant cell that has a reduced capability of adding fucose to sugar chains as compared to a corresponding wild type cell;
• and
• (b) culturing the mutant cell, thereby producing the anti-glypican 3 antibody with a modified sugar chain component, wherein the anti-glypican 3 antibody comprises a light chain variable region comprising a CDR1 comprising the amino acid sequence of SEQ ID NO:45, a CDR2 comprising the amino acid sequence of SEQ ID NO:46, and a CDR3 comprising the amino acid sequence of SEQ ID NO:60.

A method for producing an anti-glypican 3 antibody with a modified sugar chain component, the method comprising:

• (a) providing a cell expressing a nucleic acid encoding an anti-glypican 3 antibody, wherein the cell is a mutant cell that has a reduced capability of adding fucose to sugar chains as compared to a corresponding wild type cell;
• and
• (b) culturing the mutant cell, thereby producing the anti-glypican 3 antibody with a modified sugar chain component, wherein the anti-glypican 3 antibody comprises a light chain variable region comprising a CDR2 comprising the amino acid sequence of SEQ ID NO:46, a CDR3 comprising the amino acid sequence of SEQ ID NO:60, and a CDR1 comprising an amino acid sequence selected from the group consisting of SEQ ID NO:90, SEQ ID NO:91, SEQ ID NO:92, SEQ ID NO:93, SEQ ID NO:94, SEQ ID NO:95, SEQ ID NO:96, SEQ ID NO:97, SEQ ID NO:98, SEQ ID NO:99, SEQ ID NO:100, SEQ ID NO:101, SEQ ID NO:102, SEQ ID NO:103, and SEQ ID NO:104.