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US 2011/0033429 A1Published application
Inducible Gene Expression
AssigneeGeneart AG
InventorsFrank Notka, Ralf Wagner, Diana Hammer
Life of this patent
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Abstract
The invention relates to vector constructs for an HIV-specific gene therapy. The expression of transgenes is coupled with an infection of the cell with HIV while the transcription of the transgene is controlled by a transcription control region derived from HIV. In addition, the transgene is improved with regard to RNA stability and expression efficiency by modifying the nucleotide sequence.
Claims27
1-24. (canceled)
A method for inducible gene expression of a transdominant-negative (TDN) lentivirus protein in a eukaryotic cell, comprising:
- (i) modifying a target nucleic acid molecule, which codes for said TDN lentivirus protein, comprising matching codon usage to the codon usage of a mammal, wherein said modifying increases expression of said target nucleic acid molecule,
- (ii) coupling the modified target nucleic acid molecule with a promoter sequence inducible by a lentivirus Tat protein, and
- (iii) contacting said modified target nucleic acid molecule with said lentivirus Tat protein, to induce expression of the target nucleic acid molecule in a suitable expression system.
An expression vector comprising:
- (a) a target nucleic acid molecule that codes for a TDN lentivirus protein and whose codon usage is matched to that of a mammal,
- (b) a promoter sequence inducible by a lentivirus Tat protein in operative linkage with the target nucleic acid molecule, wherein the nucleic acid molecule according to (a) is modified at the nucleotide level to increase expression levels of said TDN lentivirus protein.
Dependencies are inferred from the claim text.