Method for isolating smooth muscle stem cells
AssigneeKeio University
InventorsTetsuo Maruyama, Masanori Ono
Life of this patent
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Abstract
This invention relates to a method for isolating smooth muscle stem cells derived from mammalian smooth muscle comprising bringing mammalian smooth muscle cells into contact with a fluorescence-labeled anti-CD45 antibody, anti-CD34 antibody, and anti-CD49f antibody, and isolating cells that would not bind to the anti-CD45 antibody but would bind to the anti-CD34 antibody and the anti-CD49f antibody.
Claims17
(canceled)
The method for isolating smooth muscle stem cells derived from mammalian smooth muscle, the method comprising:
- bringing the mammalian smooth muscle cells into contact with an anti-CD34 antibody and an anti-CD49f antibody labeled with fluorescent dyes;
- and
- isolating cells that bind to the anti-CD34 antibody and the anti-CD49f antibody.
A smooth muscle stem cell, isolated by the method according to claim 17, which are CD31-, CD45-, and Glycophorin A-negative and CD34- and CD49f-positive.
Dependencies are inferred from the claim text.