Modified antibodies for passive immunotherapy
OwnerZhifeng Chen, Jehangir Wadia +1inventors
InventorsZhifeng Chen, Jehangir Wadia, Robert Anthony Williamson
Life of this patent
Estimating the term…
Abstract
Provided herein are modified therapeutic antibodies containing a protein transduction domain conjugated to a neutralizing antibody. Also provided are methods for generating the modified therapeutic antibodies and methods of using the modified therapeutic antibodies for prevention and treatment of disease.
Claims53
A modified therapeutic antibody comprising:
- a protein transduction domain;
- and
- an antibody or antigen-binding fragment thereof, wherein the antigen binding domain of the antibody or antigen-binding fragment thereof specifically binds to a viral surface protein.
The modified therapeutic antibody of 1, wherein the antibody portion is AC8 (ATCC Accession No. 69522) or an antigen binding fragment thereof.
A method for increasing the therapeutic efficacy of an antiviral antibody or an antigen binding fragment thereof, comprising:
- conjugating a protein transduction domain to an antibody or antigen-binding fragment thereof wherein the antigen binding domain of the antibody or antigen-binding fragment thereof binds a glycoprotein on the surface of a virus thereby increasing the therapeutic efficacy of the antibody or antigen-binding fragment thereof compared to the therapeutic efficacy of the antibody or antigen-binding fragment thereof in the absence of the protein transduction domain,
- wherein the antibody or antigen-binding fragment is a neutralizing antibody or fragment thereof.
A protein transduction domain, wherein the protein transduction domain comprises a polypeptide selected from among:
- a) a polypeptide having an amino acid sequence B1-X1-B2-B3-B4-X2-B5-B6-X3-X4-B7, wherein:
- B1, B2, B3, B4, B5, B6, and B7 are each independently lysine or arginine;
- and
- X1, X2, X3, and X4 are each independently any amino acid except proline;
- b) a polypeptide having an amino acid sequence B1-P1-X1-B2-P2-B3-X2-X3-X4-B4, wherein:
- P1 and P2 are proline;
- B1, B2, B3 and B4 are each independently lysine or arginine;
- and
- X1, X2, X3 and X4 are each independently any amino acid;
- c) a polypeptide having an amino acid sequence B1-B2-B3-B4-B5-Q-B6-B7-Bg, wherein:
- Q is glutamine;
- and
- B1, B2, B3, B4, B5, B6, B7, and B8 are each independently lysine or arginine provided that the protein transduction domain does not have the sequence RKKRRQRRR;
- or
- d) a polypeptide having an amino acid sequence B1-B2-B3-B4-B5-X-B6-B7-B8, wherein:
- X is any amino acid except glutamine;
- and
- B1, B2, B3, B4, B5, B6, B7, and B8 are each independently lysine or arginine.
Dependencies are inferred from the claim text.