Chimeric polypeptides and their use

A method for treating a neoplastic disease comprising administering a polynucleotide encoding a chimeric polypeptide comprising:

• a) a polypeptide exhibiting affinity for specific nucleotide sequences,
• b) a DNA modifying enzyme, and
• c) a region with intracellular delivery activity,
• wherein the polypeptide exhibiting affinity for specific nucleotide sequences and the DNA modifying enzyme are derived from a type II class restriction endonuclease, a subunit or any functional fragment thereof wherein said functional fragment exhibits at least one function of the polypeptide and the DNA modifying enzyme to a subject in need thereof.

A method for treating a neoplastic disease comprising administering a vector comprising a polynucleotide encoding a chimeric polypeptide comprising:

• a) a polypeptide exhibiting affinity for specific nucleotide sequences,
• b) a DNA modifying enzyme, and
• c) a region with intracellular delivery activity,
• wherein the polypeptide exhibiting affinity for specific nucleotide sequences and the DNA modifying enzyme are derived from a type II class restriction endonuclease, a subunit or any functional fragment thereof wherein said functional fragment exhibits at least one function of the polypeptide and the DNA modifying enzyme to a subject in need thereof.

A method for treating a neoplastic disease comprising administering a cell transformed with a vector comprising a polynucleotide encoding a chimeric polypeptide comprising:

• a) a polypeptide exhibiting affinity for specific nucleotide sequences,
• b) a DNA modifying enzyme, and
• c) a region with intracellular delivery activity,
• wherein the polypeptide exhibiting affinity for specific nucleotide sequences and the DNA modifying enzyme are derived from a type II class restriction endonuclease, a subunit or any functional fragment thereof wherein said functional fragment exhibits at least one function of the polypeptide and the DNA modifying enzyme to a subject in need thereof.

A method for treating or diagnosing a neoplastic disease comprising administering a chimeric polypeptide comprising:

• a) a polypeptide exhibiting affinity for specific nucleotide sequences,
• b) a DNA modifying enzyme, and
• c) a region with intracellular delivery activity,
• wherein the polypeptide exhibiting affinity for specific nucleotide sequences and the DNA modifying enzyme are derived from a type II class restriction endonuclease, a subunit or any functional fragment thereof wherein said functional fragment exhibits at least one function of the polypeptide and the DNA modifying enzyme to a subject in need thereof.