Cysteine Engineered Antibodies For Site-Specific Conjugation
AssigneeMedlmmune, LLC
InventorsNazzareno Dimasi, Changshou Gao, Herren Wu
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Abstract
Cysteine engineered antibodies useful for the site-specific conjugation to a variety of agents are provided. Methods for the design, preparation, screening, selection and use of such antibodies are also provided.
Claims61
A cysteine engineered antibody, wherein the cysteine engineered antibody comprises a substitution of one or more amino acids to a cysteine residue in the 131-139 region of the heavy chain of an antibody as defined by the EU Index numbering system, wherein the cysteine engineered antibody comprises at least one free thiol group.
The cysteine engineered antibody of any of claim 1-29 or 55-69 wherein said antibody comprises at least a first and a second expansion of the 131-139 loop region, wherein said first expansion occurs after a position selected from the group consisting of residues 131, 132, 133, 134, 135, 136, 137, 138 and 139 and wherein said second expansion occurs after said first expansion, wherein said second expansion occurs after a position selected from the group consisting of residues 131, 132, 133, 134, 135, 136, 137, 138 and 139.
Dependencies are inferred from the claim text.