Method for measuring fluorescent light amount or light absorption amount and device for measuring the same

A method for measuring a light absorption amount, whereby a specimen is irradiated with irradiation light, and transmitted light undergoes absorption by the specimen to be measured by a light-receiving element via a receiving optical system, said method comprising the steps of:

• causing the light-receiving element to measure irradiation light at a light-amount value per specified unit area traceable to the national standard, and designated on the surface of the specimen beforehand via the receiving optical system;
• irradiating the specimen with the irradiation light;
• causing transmitted light undergoing absorption by the specimen to be measured by the light-receiving element via the receiving optical system;
• and
• computing a transmitted light amount measured by the light-receiving element on the basis of an irradiation light amount per the specified unit area traceable to the national standard, optical coefficients of the receiving optical system, and an acceptance coefficient of the light-receiving element, thereby working out a light absorption amount value per unit area traceable to the national standard.

A device for measuring a fluorescent light amount, whereby a specimen is irradiated with excitation light, and fluorescent light generated from the specimen is measured by a light-receiving element via a receiving optical system, said device comprising:

• light source means for irradiating the specimen with excitation light having a light-amount value per specified unit area traceable to the national standard, and designated on the surface of the specimen beforehand;
• light amount measuring means for causing the light-receiving element to measure the fluorescent light generated from the specimen via the receiving optical system;
• and
• fluorescent light amount computing means for computing a fluorescent light-amount as measured by the light-receiving element via the receiving optical system on the basis of an excitation light-amount per the specified unit area, optical coefficients of the receiving optical system, and the acceptance coefficient of the light-receiving element, thereby working out a light amount value per the unit area traceable to the national standard.

A device for measuring a light absorption amount, whereby a specimen is irradiated with irradiation light, and transmitted light undergoes absorption by the specimen to be measured by a light-receiving element via a receiving optical system, said method comprising the steps of:

• light source means for irradiating the specimen with the irradiation light having a light-amount value per specified unit area traceable to the national standard, and designated on the surface of the specimen beforehand;
• light amount measuring means for causing irradiation light emitted from the light source means and transmitted light undergoing absorption by the specimen to be measured by the light-receiving element via the receiving optical system;
• and
• light absorption amount computing means for computing a transmitted light amount measured by the light-receiving element on the basis of an irradiation light amount per the specified unit area traceable to the national standard, optical coefficients of the receiving optical system, and an acceptance coefficient of the light-receiving element, thereby working out a light absorption amount value per unit area traceable to the national standard.