Altering the interface of hydrocarbon-coated surfaces
AssigneeE. I. Du Pont de Nemours and Company
InventorsERIC R. CHOBAN, ROBERT D. FALLON, EDWIN R. HENDRICKSON, SCOTT CHRISTOPHER JACKSON, ABIGAIL K. LUCKRING, MICHAEL P. PERRY, DAVID ALAN SUCHANEC, SHERYL M. ANYZEK
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Abstract
Methods and compositions are provided wherein microorganisms are used to alter the interface of hydrocarbons and hydrocarbon-coated surfaces to increase oil recovery, for improved bioremediation and/or pipeline maintenance.
Claims39
A method for altering the wettability of a hydrocarbon coated surface comprising:
- a) providing a hydrocarbon-coated surface;
- b) providing a medium selected from the group consisting of:
- i) a cell containing medium comprising one or more Shewanella sp.;
- and
- ii) a conditioned medium which is substantially cell free and which has been in contact with one or more Shewanella sp.;
- c) contacting said hydrocarbon-coated surface with the medium of b) wherein the medium alters the wettability of said hydrocarbon-coated surface.
A method for oil recovery from an oil reservoir comprising:
- a) providing one or more Shewanella sp;
- b) injecting an oil reservoir with the one or more Shewanella sp of (a);
- and
- c) injecting said oil reservoir with a nutrient solution comprising one or more electron acceptors selected from the group consisting of nitrate, fumarate, ferric ion, manganese (MnIV) ion and mixtures thereof;
- wherein said one or more Shewanella sp. grow under anaerobic conditions in the oil reservoir and said growth promotes improved oil recovery.
A composition for enhanced oil recovery comprising:
- a) a Shewanella sp having a 16S rDNA that has at least 95% identity to the 16S rDNA sequence as set forth in any of SEQ ID NO.'s 3, 5, 15, 16 and 17;
- and
- b) an electron acceptor selected from the group consisting of nitrate, fumarate, ferric ion, manganese (MnIV) ion and mixtures thereof.
A Shewanella sp. having the following characteristics:
- a) a 16S rDNA comprising signature sequences SEQ ID NOs:
- 13 and 14;
- and
- b) riboprint pattern identifier of 212-824-S-4 as illustrated in FIG. 16;
- wherein 16S rDNA of (a) has at least about 97% identity to the 16S rDNA sequence as set forth in SEQ ID NO:3.
Dependencies are inferred from the claim text.