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US 2011/0020854 A1Published application
Enhancing Endotoxin Detection
AssigneeBioDtech, Inc.
InventorsMichael G. Pepe, Milton Keith Champion
Life of this patent
Estimating the term…
Abstract
Provided herein are methods for detecting gram negative bacteria or lipopolysaccharide in a sample. Kits for detecting gram negative bacteria or lipopolysaccharide in a sample are provided.
Claims4
1-28. (canceled)
A method for detecting gram negative bacteria or lipopolysaccharide in a sample comprising the steps of:
- (a) contacting the sample with an active acidic protease under conditions that result in degradation of the proteins in the sample;
- (b) further contacting the sample with an amebocyte lysate;
- and
- (c) determining whether a gelation reaction occurs in the amebocyte lysate, a gelation reaction indicating the sample contains gram negative bacteria or lipopolysaccharide.
1 more element
31-60. (canceled)
Dependencies are inferred from the claim text.