Microfluidic cartridge with solution reservoir-pump chamber

A microfluidic cartridge with solution reservoir-pump chamber comprising:

• a channel-chamber layer, a sealing layer for the channel-chamber layer, a printed circuit board bound with magnetoresistive biochip, and at least one fluid inlet, characterized in that:
• the channel-chamber layer is provided with a waste reservoir, a reaction-detection chamber, at least one solution reservoir-pump chamber and at least one micro-channel wherein the waste reservoir has a waste outlet and communicates with the reaction-detection chamber via the micro-channel, and the other port of the reaction-detection chamber communicates with said solution reservoir-pump chamber and said fluid inlet via said micro-channel;
• said solution reservoir-pump chamber includes a sample reservoir and a reagent reservoir with each consists of a cylindrical chamber and an elastic element used for sealing and pumping the liquid in said cylindrical chamber;
• the sealing layer and the channel-chamber layer are aligned and sealed so as to completely form the waste reservoir, the reaction-detection chamber, the sealable solution inlet, the solution reservoir-pump chamber and the micro-channel;
• a chip window is formed in a position of the sealing layer corresponding to the position of the reaction-detection chamber in said channel-chamber layer, wherein said chip window is aligned and sealed between said reaction-detection chamber and said magnetoresistive biochip on said printed circuit board to form a complete reaction-detection chamber with the surface of said magnetoresistive chip as the reaction and detection surface;
• and
• the surface of the magnetoresistive chip is bio-functionalized to form a biochip which is connected to a testing apparatus outside said microfluidic cartridge by a plurality of electrically conductive lines on the printed circuit board.