Methods involving graf polypeptides
OwnerGary J. Doherty, Richard Lundmark +1inventors
InventorsGary J. Doherty, Richard Lundmark, Harvey T. McMahon
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Abstract
The invention relates to a method of identifying a modulator of clathrin-independent endocytosis, said method comprising (i) providing a GRAF protein, said GRAF protein comprising a GAP domain; (ii) providing a candidate modulator; and (iii) determining the effect of said candidate modulator on the GAP activity of said GRAF protein, wherein a change in GAP activity of said GRAF protein in the presence of said candidate modulator identifies said candidate modulator as a modulator of clathrin-independent endocytosis. The invention also relates to GRAF proteins and to methods of manufacture of GRAF modulators.
Claims23
A method of identifying a modulator of clathrin-independent endocytosis, said method comprising
- (i) providing a GRAF protein, said GRAF protein comprising a GAP domain;
- (ii) providing a candidate modulator;
- and
- (iii) determining an effect of said candidate modulator on GAP activity of said GRAF protein,
- wherein a change in the GAP activity of said GRAF protein in the presence of said candidate modulator identifies said candidate modulator as a modulator of clathrin-independent endocytosis.
19-20. (canceled)
A composition comprising a GRAF polypeptide having a mutation at an amino acid residue corresponding to amino acid 412 of human GRAF1.
(canceled)
A method for treating solid cell malignancy cancer or immuno suppressing a subject comprising:
- administering to the subject a modulator of clathrin-independent endocytosis.
Dependencies are inferred from the claim text.