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US 2010/0331193 A1Published application
Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
AssigneeMaxygen, Inc.
InventorsWillem P.C. Stemmer, Andreas Crameri
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Abstract
Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.
Claims2
A method for evolving a protein encoded by a DNA substrate molecule comprising:
- (a) digesting at least a first and second DNA substrate molecule, wherein the at least a first and second substrate molecules differ from each other in at least one nucleotide, with a restriction endonuclease;
- (b) ligating the mixture to generate a library of recombinant DNA molecules;
- (c) screening or selecting the products of (b) for a desired property;
- and
- (d) recovering a recombinant DNA substrate molecule encoding an evolved protein.
2 more elements
2-273. (canceled)
Dependencies are inferred from the claim text.