Mutants Having Capability To Produce 1, 4-Butanediol And Method For Preparing 1, 4-Butanediol Using The Same
AssigneesLG Chem, Ltd., Korea Advanced Institute of Science and Technology
InventorsSi Jae Park, Sang Hyun Lee, Sang Yup Lee, Eun Jeong Lee
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Abstract
A mutant capable of producing 1,4-butanediol and a method of preparing 1,4-butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process.
Claims32
A mutant exhibiting high production of 1,4-butanediol, which is prepared by introducing or amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate, and 4-hydroxybutyrate into 1,4-butanediol, in a microorganism capable of producing succinate.
A mutant exhibiting high production of 1,4-butanediol, which is prepared by introducing or amplifying a gene encoding Cat1;
- a gene encoding SucD;
- a gene encoding 4hbD or GHB;
- a gene encoding 4-hydroxybutyrate-CoA transferase, or a gene encoding Ptb and a gene encoding Buk;
- and a gene encoding butyl-CoA dehydrogenase, in a microorganism capable of producing succinate.
A butyl-CoA dehydrogenase gene having a base sequence of SEQ ID NO:
- 8 or 9.
Dependencies are inferred from the claim text.