Method for producing vaccinal viral strain of a virus of the reoviridae family
AssigneeLondon School of Hygiene and Tropical Medicine
InventorsPolly Roy, Mark Boyce
Life of this patent
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Abstract
The invention relates to a method for producing a modified viral strain of a virus which is a member of the Reoviridae family and, in particular, relates to vaccinal viral strains of the Orbivirus genus.
Claims34
A method for producing a vaccinal viral strain of a virus which is a member of the Reoviridae family, the method comprising:
- introducing a mutation into the Reoviridae virus so that the function of an essential gene is destroyed;
- transfecting a cell with viral single stranded RNA (ssRNA) derived from the virus genome and comprising the mutation;
- and
- culturing the transfected cell under suitable conditions in order to lead to the production of the vaccinal viral strain,
- wherein the cell complements the function of the essential viral gene thereby allowing the vaccinal viral strain to replicate in the cell.
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-26. (canceled)
A screening method for identifying essential genes of a virus which is a member of the Reoviridae family, the method comprising:
- introducing a mutation into the Reoviridae virus so that the function of a gene is destroyed;
- transfecting a cell with viral ssRNA derived from the VIrUS genome and comprising the mutation;
- and
- culturing the transfected cell under suitable conditions, wherein the viral gene is not an essential gene if the virus produced after culturing the transfected cell is pathogenic.
A method for producing a modified viral strain of a virus which is a member of the Reoviridae family, the method comprising:
- introducing a modification into the Reoviridae virus;
- transfecting a cell with viral single stranded RNA (ssRNA) derived from the virus genome and comprising the modification;
- and
- culturing the transfected cell under suitable conditions in order to lead to the production of the modified viral strain,
- wherein the cell allows the modified viral strain to replicate in the cell.
Dependencies are inferred from the claim text.