Production of hybrid lactuca sativa seeds
AssigneeVilmorin
InventorHervé Michel
Life of this patent
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Abstract
The present invention concerns hybrid Lactuca sativa seeds which have a male sterile genotype and which are heterozygous for at least one distinct gene which provides plants derived from said seeds with a distinct phenotype. The invention also concerns a method for producing hybrid Lactuca sativa seeds, comprising a step for culture, in an enclosed medium, of phenotypically male sterile plants and phenotypically male fertile plants, a step for pollinization by diptera introduced into the closed medium upon flowering of the plants, and a step for harvesting the seeds produced by the male sterile plants.
Claims29
A hybrid Lactuca sativa seed, characterized in that it:
- has a male sterile genotype;
- and
- is heterozygous for at least one distinct gene, providing the plant derived from said seed with a detectable phenotype.
A hybrid Lactuca sativa plant, characterized in that it:
- has a heterozygous male sterile genotype;
- and
- is heterozygous for at least one distinct gene, providing it with a detectable phenotype.
A cell from a hybrid Lactuca sativa plant, characterized in that it:
- has a heterozygous male sterile genotype;
- and
- is heterozygous for at least one distinct gene, providing it with a phenotype which is detectable in the plant.
(canceled)
Use of insects of the diptera order in a concentration of more than 100 diptera per m2 or 25 diptera per m3 to carry out pollinization in an enclosed medium of male sterile Lactuca sativa plants by male fertile plants.
27-30. (canceled)
A method for obtaining hybrid Lactuca sativa seeds, comprising:
- a step for culture, in an enclosed medium, of phenotypically male sterile Lactuca sativa plants used as “female” parents and of phenotypically male fertile Lactuca sativa plants used as “male” parents in proximity to each other, one of the two parents being, as a supplemental characteristic, homozygous for a gene which provides it with a detectable phenotype other than male sterility, the other parent not carrying said gene;
- a step for pollinization using diptera introduced into the enclosed medium at the time of flowering of the plants in a concentration of more than 100 diptera per m2 or more than 25 diptera per m3, preferably at least 250 diptera per m2 or at least 75 diptera per m3;
- and
- a step for harvesting the seeds produced by the male sterile plants.
32-47. (canceled)
Dependencies are inferred from the claim text.