Nucleotide sequences and corresponding polypeptides conferring modulated growth rate and biomass in plants grown in saline conditions
AssigneeCeres, Inc.
InventorsFasong Zhou, Julissa Sosa
Life of this patent
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Abstract
The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides able confer the trait of improved plant size, vegetative growth, growth rate, seedling vigor and/or biomass in plants challenged with saline conditions. The present invention further relates to the use of these nucleic acid molecules and polypeptides in making transgenic plants, plant cells, plant materials or seeds of a plant having plant size, vegetative growth, growth rate, seedling vigor and/or biomass that are improved in saline conditions with respect to wild-type plants grown under similar conditions
Claims20
A method of producing a plant or plant tissue, said method comprising growing a plant cell comprising an exogenous nucleic acid, said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence encoding a polypeptide, wherein the HMM bit score of the amino acid sequence of said polypeptide is greater than 50, said HMM based on the amino acid sequences depicted in one of FIGS. 1, 2 and 3, and wherein said plant or plant tissue has a difference in the level of salt tolerance as compared to the corresponding level in a control plant or plant tissue that does not comprise said nucleic acid.
A method of producing a plant or plant tissue, said method comprising growing a plant cell comprising an exogenous nucleic acid, said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence encoding a polypeptide having 85 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 81, 83-87, 89-95, 97-99, 103, 105, 107, 109, 111-114, 116-120, 122 and 124-128, wherein a plant or plant tissue produced from said plant cell has a difference in the level of salt tolerance as compared to the corresponding level in a control plant or plant tissue that does not comprise said nucleic acid.
A method of producing a plant, said method comprising growing a plant cell comprising an exogenous nucleic acid, said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence having 85 percent or greater sequence identity to a nucleotide sequence selected from the group consisting of SEQ ID NOs:
- 79-80, 82, 88, 96, 100-102, 104, 106, 108, 110, 115, 121 and 123, wherein a plant or plant tissue produced from said plant cell has a difference in the level of salt tolerance as compared to the corresponding level in a control plant or plant tissue that does not comprise said nucleic acid.
A method of modulating the level of salt tolerance in a plant, said method comprising introducing into a plant cell an exogenous nucleic acid, said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence encoding a polypeptide, wherein the HMM bit score of the amino acid sequence of said polypeptide is greater than 50, said HMM based on the amino acid sequences depicted in one of FIGS. 1, 2 and 3, and wherein a plant or plant tissue of a plant produced from said plant cell has a difference in the level of salt tolerance as compared to the corresponding level in a control plant or plant tissue that does not comprise said exogenous nucleic acid.
A method of modulating the level of salt tolerance in a plant, said method comprising introducing into a plant cell an exogenous nucleic acid, said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence encoding a polypeptide having 85 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 81, 83-87, 89-95, 97-99, 103, 105, 107, 109, 111-114, 116-120, 122 and 124-128, wherein a plant or plant tissue produced from said plant cell has a difference in the level of salt tolerance as compared to the corresponding level in a control plant or plant tissue that does not comprise said nucleic acid.
A method of modulating the level of salt tolerance in a plant, said method comprising introducing into a plant cell an exogenous nucleic acid, said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence having 80 percent or greater sequence identity to a nucleotide sequence selected from the group consisting of SEQ ID NOs:
- 79-80, 82, 88, 96, 100-102, 104, 106, 108, 110, 115, 121 and 123, wherein a plant or plant tissue produced from said plant cell has a difference in the level of salt tolerance as compared to the corresponding level in a control plant or plant tissue that does not comprise said nucleic acid.
A plant cell comprising an exogenous nucleic acid said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence encoding a polypeptide, wherein the HMM bit score of the amino acid sequence of said polypeptide is greater than 50, said HMM based on the amino acid sequences depicted in one of FIGS. 1, 2 and 3, and wherein said plant cell has a difference in the level of salt tolerance as compared to the corresponding level in a control plant that does not comprise said nucleic acid.
A plant cell comprising an exogenous nucleic acid said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 81, 83-87, 89-95, 97-99, 103, 105, 107, 109, 111-114, 116-120, 122 and 124-128, wherein a plant produced from said plant cell has a difference in the level of salt tolerance as compared to the corresponding level in a control plant that does not comprise said nucleic acid.
A plant cell comprising an exogenous nucleic acid said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence having 80 percent or greater sequence identity to a nucleotide sequence selected from the group consisting of SEQ ID NOs:
- 79-80, 82, 88, 96, 100-102, 104, 106, 108, 110, 115, 121 and 123, wherein a plant produced from said plant cell has a difference in the level of salt tolerance as compared to the corresponding level in a control plant that does not comprise said nucleic acid.
An isolated nucleic acid comprising a nucleotide sequence having 85% or greater sequence identity to one of the nucleotide sequences set forth in SEQ ID NO:
- 82, 102, 104, 106, 110, 115 and 121.
An isolated nucleic acid comprising a nucleotide sequence encoding a polypeptide having 85% or greater sequence identity to one of the amino acid sequences set forth in SEQ ID NO:
- 83, 87, 103, 105, 107, 111, 116 and 122.
A method of identifying whether a genetic polymorphism is associated with variation in a trait, said method comprising:
- a) determining whether one or more genetic polymorphisms in a population of plants is associated with the locus for a polypeptide selected from the group consisting of the polypeptides depicted in FIGS. 1, 2 and 3 and functional homologs thereof;
- and
- b) measuring the correlation between variation in said trait in plants of said population and the presence of said one or more genetic polymorphisms in plants of said population, thereby identifying whether or not said one or more genetic polymorphisms are associated with variation in said trait.
A method of making a plant line, said method comprising:
- a) determining whether one or more genetic polymorphisms in a population of plants is associated with the locus for a polypeptide selected from the group consisting of the polypeptides depicted in FIGS. 1, 2 and 3 and functional homologs thereof;
- b) identifying one or more plants in said population in which the presence of at least one allele at said one or more genetic polymorphisms is associated with variation in a salt tolerance trait;
- c) crossing each said one or more identified plants with itself or a different plant to produce seed;
- d) crossing at least one progeny plant grown from said seed with itself or a different plant;
- and
- e) repeating steps c) and d) for an additional 0-5 generations to make said plant line, wherein said at least one allele is present in said plant line.
Dependencies are inferred from the claim text.