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US 2010/0305312 A1Published application
Method For The Extraction And Purification Of Nucleic Acids On A Membrane
AssigneeMillipore Corporation
InventorsRoxana Isac, Frederic Marc
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Abstract
The invention relates to a method for the extraction and detection of nucleic acids on a membrane, making it possible to identify the microorganisms present in low concentration in a liquid or gaseous medium, as well as a novel lysis composition comprising guanidium chloride and between 0.1 and 1% N-Lauroyl-Sarcosine (NLS) making it possible to implement this method.
Claims9
Method for the extraction of the nucleic acids of one or more cells, comprising the following steps:
- a) the cells are treated with guanidium chloride and N-Lauroyl-sarcosine (NLS) in solution, in order to lyse the cells and release the nucleic acids that they contain;
- b) the nucleic acids are separated from the cell lysate obtained in a) by filtration of said lysate across a membrane;
- c) the nucleic acids retained by said membrane are recovered in solution in water or a weakly ionized aqueous solution.
Dependencies are inferred from the claim text.