Low molecular weight heparin composition and uses thereof

A method of making a low molecular weight heparin (LMWH) composition, the method

• comprising
• providing a precursor LMWH composition having an average chain length of about 8 to 14 disaccharides obtained by salt precipitation and cleavage of a heparin sample with an enzyme which cleaves at unsulfated uronic acid linkages;
• and
• subjecting the precursor LMWH composition to a size selection step to obtain a LMWH composition having an average chain length (n) of about 9 to 16 disaccharides, wherein the LMWH composition comprises:
• oligosaccharide chains that have the following structure:
• and oligosaccharide chains that have the following structure:
• wherein
• R is H or SO3Na;
• R1 is SO3Na or COCH3;
• n=2-50;
• and wherein the composition has the following properties:
• (g) 5 to 15% ΔUHNAc,6SGHNS,3S,6S as measured by mole %,
• (h) weight average molecular weight of 5500 to 8500 Da,
• (i) anti-Xa activity of 120 to 380 IU/mg,
• (j) an anti-Xa to anti-IIa ratio of 2:1 to 1:1, and
• (k) the ratio of (d) is constant over a period of about 30 to 120 minutes after administration to a subject.