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US 2010/0304998 A1Published application
Chemical Proteomic Assay for Optimizing Drug Binding to Target Proteins
AssigneeMarquette University
InventorDaniel S. Sem
Life of this patent
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Abstract
Disclosed herein are methods related to drug development. The methods typically include steps whereby an existing drug is modified to obtain a derivative form or whereby an analog of an existing drug is identified in order to obtain a new therapeutic agent that preferably has a higher efficacy and fewer side effects than the existing drug.
Claims20
A method comprising:
- (a) passing a biological sample comprising a target protein and optionally a non-target protein over a column, the column comprising an affinity resin for the target protein, the affinity resin comprising a resin conjugated or covalently attached to a first chemical compound that binds to the target protein;
- (b) washing the column and removing proteins that are not bound to the affinity resin;
- (c) eluting proteins from the column that are bound to the affinity resin by passing a solution comprising a second chemical compound over the column;
- and
- (d) identifying proteins in the eluate, thereby obtaining a proteomic profile for the second chemical compound.
2 more elements
A method comprising:
- (a) passing a biological sample comprising proteins over columns comprising a chemical-resin library, wherein each column comprises a separate member of the chemical-resin library and the chemical-resin library comprises a separate chemical compound conjugated to a resin;
- (b) washing each column to remove any non-bound proteins;
- (c) eluting any bound proteins from each column;
- and
- (d) identifying proteins in the eluates, thereby generating a proteomic profile for each column, and optionally comparing the proteomic profiles for two or more columns.
2 more elements
A method comprising:
- (a) passing a biological sample comprising a target protein and a non-target protein over a first column, the first column comprising an affinity resin for the target protein, the affinity resin comprising a resin conjugated or covalently attached to a first chemical compound that binds to the target protein;
- (b) washing the first column and removing proteins that are not bound to the affinity resin;
- (c) eluting proteins from the first column that are bound to the affinity resin;
- (d) identifying proteins in the eluate including the target protein and optionally the non-target protein;
- (e) passing the biological sample comprising the target protein and the non-target protein over a second column, the second column comprising an affinity resin for the target protein, the affinity resin comprising a resin conjugated or covalently attached to a second chemical compound that binds to the target protein;
- (f) washing the second column and removing proteins that are not bound to the affinity resin;
- (g) eluting proteins from the second column that are bound to the affinity resin;
- and
- (h) identifying proteins in the eluate including the target protein and optionally the non-target protein.
6 more elements
Dependencies are inferred from the claim text.