Method of selecting aptamers
AssigneePronota N.V.
InventorClive Gavin Brown
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Abstract
The present invention is a method for the identification of one or more aptamers to at least one target molecule, the method comprising: selecting candidate aptamer sequences that bind to a target molecule, assigning to the bound sequences a measure (fitness function) of each sequence's aptameric potential, allowing evolution of some or all of the sequences to create a new mixture of candidate sequences, and repeating the method with the newly created candidate aptamer pool until the aggregate aptameric potential of the candidate pool reaches a plateau, wherein sequences present in the final pool are optimal aptamers to the target molecule.
Claims30
A method for the identification of one or more aptamers to at least one target molecule, the method comprising:
- a) contacting a pool of candidate polymer sequences with at least one target molecule;
- b) partitioning unbound sequences from those sequences that have bound specifically to target molecule(s);
- c) dissociating the sequence-target complexes to obtain a ligand-enriched mixture of sequences;
- d) assigning to each sequence obtained in step c) a measure (fitness function) of the sequence's aptameric potential;
- e) using the measure of step d) to determine the aptameric potential of the ligand-enriched mixture;
- f) allowing evolution of some or all of the sequences obtained in step c) using information obtained in step e) to create a new mixture of sequences;
- and
- g) repeating the steps a) to f) with the newly created candidate aptamer pool until the aggregate aptameric potential of the candidate pool reaches a plateau, wherein sequences present in the final pool are optimal aptamers to the at least one target molecule.
Dependencies are inferred from the claim text.