Methods and kits for diagnosis and/or prognosis of the tolerant state in liver transplantation

A method of assessing diagnosis and/or prognosis of the tolerant state in liver transplantation in a human patient, comprising the steps of:

• (a) obtaining a biological sample from the patient;
• and
• (b) measuring the expression levels in the sample of a set of genes comprising the following twenty two:
• transforming growth factor beta receptor III (TGFBR3, NCBI Gene ID 7049), killer cell lectin-like receptor subfamily B member 1 (KLRB1, NCBI Gene ID 3820), asparagine-linked glycosylation 8 homolog (ALG8, NCBI Gene ID 79053), Fanconi anemia complementation group G (FANCG, NCBI Gene ID 2189), gem associated protein 7 (GEMIN7, NCBI Gene ID 79760), natural killer cell group 7 sequence (NKG7, NCBI Gene ID 4818), RAD23 homolog B of Saccharomyces cerevisiae (RAD23B, NCBI Gene ID 5887), SLAM family member 7 (SLAMF7, NCBI Gene ID 57823), TP53 regulated inhibitor of apoptosis 1 (TRIAP1, NCBI Gene ID 51499), protein phosphatase 1B magnesium-dependent beta isoform (PPM1B, NCBI Gene ID 5495), chromosome 10 open reading frame 119 (C10orf119, NCBI Gene ID 79892), T cell receptor delta locus (TRD@, NCBI Gene ID 6964), nucleolar protein family A member 1 (NOLA1, NCBI Gene ID 54433), DCN1 defective in cullin neddylation 1 domain containing 1 of Saccharomyces cerevisiae (DCUN1D1, NCBI Gene ID 54165), dystrobrevin binding protein 1 (DTNBP1, NCBI Gene ID 84062), N-acetylglucosamine-1-phosphate transferase alpha and beta subunits (GNPTAB, NCBI Gene ID 79158), proteasome 26S subunit non-ATPase 14 (PSMD14, NCBI Gene ID 10213), coatomer protein complex subunit zeta 1 (COPZ1, NCBI Gene ID 22818), S100 calcium binding protein A10 (S100A10, NCBI Gene ID 6281), ataxin 10 (ATXN10, NCBI Gene ID 25814), G-rich RNA sequence binding factor 1 (GRSF1, NCBI Gene ID 2926), and CD244 molecule natural killer cell receptor 2B4 (CD244, NCBI Gene ID 51744);
• wherein the corresponding gene expression levels above or below predetermined cut-off levels are indicative of the tolerant state in liver transplantation.