Methods and compositions for determining whether a subject carries a disease associated gene mutation common in jewish populations
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsIris Schrijver, Phyllis Gardner, Eugene Pergament, Morry Fiddler
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Abstract
Methods are provided for determining whether a subject carries a disease associated gene mutation common in Jewish populations. In practicing the subject methods, an array comprising a plurality of associated gene mutation probes is contacted with a nucleic acid sample from the subject, and the presence of any resultant surface bound target nucleic acids is detected to determine whether the subject carries an disease associated gene mutation common in Jewish populations. In addition, reagents and kits thereof that find use in practicing the subject methods are provided.
Claims28
A method of determining whether a subject carries a disease associated gene mutation common in Jewish populations, said method comprising:
- (a) contacting an array comprising a plurality of distinct nucleic acid disease associated gene mutation probes immobilized on a surface of a solid support with a nucleic acid sample from said subject to produce a sample contacted array;
- (b) contacting said sample contacted array with a polymerase and at least two different distinguishably labeled dideoxynucleotides under primer extension conditions;
- and
- (c) detecting the presence of any resultant terminally labeled nucleic acids immobilized on said substrate surface to determine whether said subject carries a disease associated gene mutation common in Jewish populations.
An array comprising a plurality of at about 25 or more distinct nucleic acid disease associated gene mutation probes immobilized on a surface of a solid support.
A method of determining whether a subject carries a disease associated gene mutation common in Jewish populations, said method comprising:
- (a) contacting an array comprising a plurality of about 25 or more distinct nucleic acid disease associated gene mutation probes immobilized on a surface of a solid support with a nucleic acid sample of target nucleic acids from said subject to produce a sample contacted array;
- (b) detecting the presence of any resultant target nucleic acids immobilized on said substrate surface to determine whether said subject carries a disease associated gene mutation common in Jewish populations.
A kit for use determining whether a subject carries a disease associated gene mutation common in Jewish populations, said kit comprising:
- (a) an array comprising a plurality of about 25 distinct nucleic acid disease associated gene mutation probes immobilized on a surface of a solid support;
- and
- (b) at least two different distinguishably labeled dideoxynucleotides (ddNTPs).
A method of determining whether any of a plurality of subjects carry a disease associated gene mutation common in Jewish populations, said method comprising:
- (a) producing a plurality of nucleic acid samples from said plurality of subjects, wherein each of said plurality of nucleic acid samples corresponds to one of said plurality of subjects;
- (b) contacting each of said plurality of nucleic acid samples with an array comprising a plurality of distinct nucleic acid disease associated gene mutation probes immobilized on a surface of a solid support to produce a plurality of sample contacted arrays;
- (c) contacting each of said plurality of sample contacted arrays with a polymerase and at least two different distinguishably labeled dideoxynucleotides under primer extension conditions;
- and
- (d) detecting the presence of any resultant terminally labeled nucleic acids immobilized on said substrate surface to determine whether any of said plurality of subjects carry a disease associated gene mutation common in Jewish populations.
Dependencies are inferred from the claim text.