US 2010/0304984 A1Published application

Prediction of potential drug-drug interactions using gene expression profiling of drug transporters, cytochrome p450s and nuclear x receptors

AssigneeNoab Biodiscoveries Inc.

InventorsRobert Shipman, Jodi A Morrison, Ines De Lannoy

Life of this patent

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Abstract

The invention provides materials and methods for detecting the expression of genes encoding cytochrome p450, nuclear X receptors, phase H transferases, and solute carrier family uptake pumps. The materials include sets of primers, PCR amplicons and arrays. The methods of the invention include hybridization assays. Kits and assays for the detection of the expression of the genes are also provided by the invention. In addition, the invention provides the use of the materials and methods of the invention in drug screening assays.

Claims56

Dependent claims

An array comprising two or more nucleic acid molecules immobilized on a substrate, wherein at least two of the nucleic acid molecules have a nucleic acid sequence consisting of:

(a) a nucleic acid sequence as shown in SEQ ID NOS:
4, 8, 12, 16, 20, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104, 108, 112, 116, 120, 124, 128, 132, 136, 140, 144, 148, 152, 156, 160, 164, 168, 172, 176, 180, 184, 188, 192, 196, 200, 204, 208, 212, 216, 220, 224, 228, 232, 236, 240, 244, 248, 252, 256, 260, 264, 268, 272, 276, 280, 284, or 288;
(b) a nucleic acid sequence prepared using amplification and primer pairs, wherein the primer pairs are selected from the following pairs of nucleic acid sequences:
SEQ ID NO:1 and SEQ ID NO:2;
SEQ ID NO:5 and SEQ ID NO:6;
SEQ ID NO:9 and SEQ ID NO:10;
SEQ ID NO:13 and SEQ ID NO:14;
SEQ ID NO:17 and SEQ ID NO:18;
SEQ ID NO:21 and SEQ ID NO:22;
SEQ ID NO:25 and SEQ ID NO:26;
SEQ ID NO:29 and SEQ ID NO:30;
SEQ ID NO:33 and SEQ ID NO:34;
SEQ ID NO:37 and SEQ ID NO:38;
SEQ ID NO:41 and SEQ ID NO:42;
SEQ ID NO:45 and SEQ ID NO:46;
SEQ ID NO:49 and SEQ ID NO:50;
SEQ ID NO:53 and SEQ ID NO:54;
SEQ ID NO:57 and SEQ ID NO:58;
SEQ ID NO:61 and SEQ ID NO:62;
SEQ ID NO:65 and SEQ ID NO:66;
SEQ ID NO:69 and SEQ ID NO:70;
SEQ ID NO:73 and SEQ ID NO:74;
SEQ ID NO:77 and SEQ ID NO:78;
SEQ ID NO:81 and SEQ ID NO:82;
SEQ ID NO:85 and SEQ ID NO:86;
SEQ ID NO:89 and SEQ ID NO:90;
SEQ ID NO:93 and SEQ ID NO:94;
SEQ ID NO:97 and SEQ ID NO:98;
SEQ ID NO:101 and SEQ ID NO:102;
SEQ ID NO:105 and SEQ ID NO:106;
SEQ ID NO:109 and SEQ ID NO:110;
SEQ ID NO:113 and SEQ ID NO:114;
SEQ ID NO:117 and SEQ ID NO:118;
SEQ ID NO:121 and SEQ ID NO:122;
SEQ ID NO:125 and SEQ ID NO:126;
SEQ ID NO:129 and SEQ ID NO:130;
SEQ ID NO:133 and SEQ ID NO:134;
SEQ ID NO:137 and SEQ ID NO:
138;
SEQ ID NO:141 and SEQ ID NO:142;
SEQ ID NO:145 and SEQ ID NO:146;
SEQ ID NO:149 and SEQ ID NO:150;
SEQ ID NO:153 and SEQ ID NO:154;
SEQ ID NO:157 and SEQ ID NO:158;
SEQ ID NO:161 and SEQ ID NO:162;
SEQ ID NO:165 and SEQ ID NO:166;
SEQ ID NO:169 and SEQ ID NO:170;
SEQ ID NO:173 and SEQ ID NO:174;
SEQ ID NO:177 and SEQ ID NO:178;
SEQ ID NO:181 and SEQ ID NO:182;
SEQ ID NO:185 and SEQ ID NO:186;
SEQ ID NO:189 and SEQ ID NO:190;
SEQ ID NO:193 and SEQ ID NO:194;
SEQ ID NO:197 and SEQ ID NO:198;
SEQ ID NO:201 and SEQ ID NO:202;
SEQ ID NO:205 and SEQ ID NO:206;
SEQ ID NO:209 and SEQ ID NO:210;
SEQ ID NO:213 and SEQ ID NO:214;
SEQ ID NO:217 and SEQ ID NO:218;
SEQ ID NO:221 and SEQ ID NO:222;
SEQ ID NO:225 and SEQ ID NO:226;
SEQ ID NO:229 and SEQ ID NO:230;
SEQ ID NO:233 and SEQ ID NO:234;
SEQ ID NO:237 and SEQ ID NO:238;
SEQ ID NO:241 and SEQ ID NO:242;
SEQ ID NO:245 and SEQ ID NO:246;
SEQ ID NO:249 and SEQ ID NO:250;
SEQ ID NO:253 and SEQ ID NO:254;
SEQ ID NO:257 and SEQ ID NO:258;
SEQ ID NO:261 and SEQ ID NO:262;
SEQ ID NO:265 and SEQ ID NO:266;
SEQ ID NO:269 and SEQ ID NO:270;
SEQ ID NO:273 and SEQ ID NO:274;
SEQ ID NO:277 and SEQ ID NO:278;
SEQ ID NO:281 and SEQ ID NO:282;
or
SEQ ID NO:285 and SEQ ID NO:286;
(c) a nucleic acid sequence in (a) or (b) wherein T can also be U;
or
(d) a fragment of (a) to (c).

77 more elements

An array for screening a sample for the presence of nucleic acid molecules that encode cytochrome P450 enzymes, uptake transporters and/or nuclear xenoreceptors, the array comprising a substrate having immobilized in distinct spots thereon at least 2 nucleic acid probes selected from the group consisting of:

1) a probe that specifically hybridizes to a nucleic acid sequence encoding CYP1A2, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:4,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:1 and SEQ ID NO:2,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
2) a probe that specifically hybridizes to a nucleic acid sequence encoding CYP1B1, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:8,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:5 and SEQ ID NO:6,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
3) a probe that specifically hybridizes to a nucleic acid sequence encoding CYP2A6, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:12,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:9 and SEQ ID NO:10,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
4) a probe that specifically hybridizes to a nucleic acid sequence encoding CYP2B6, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:16,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:13 and SEQ ID NO:14,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
5) a probe that specifically hybridizes to a nucleic acid sequence encoding CYP2C8 variant 1, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:20,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:17 and SEQ ID NO:18,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
6) a probe that specifically hybridizes to a nucleic acid sequence encoding CYP2C8 variant 2, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:24,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:21 and SEQ ID NO:22,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
7) a probe that specifically hybridizes to a nucleic acid sequence encoding CYP2C9, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:28,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:25 and SEQ ID NO:26,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
8) a probe that specifically hybridizes to a nucleic acid sequence encoding CYP2C19, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:32,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:29 and SEQ ID NO:30,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
9) a probe that specifically hybridizes to a nucleic acid sequence encoding CYP2D6, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:36,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:33 and SEQ ID NO:34,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
10) a probe that specifically hybridizes to a nucleic acid sequence encoding CYP2E1, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:40,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:37 and SEQ ID NO:38,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
11) a probe that specifically hybridizes to a nucleic acid sequence encoding CYP3A4, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:44,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:41 and SEQ ID NO:42,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
12) a probe that specifically hybridizes to a nucleic acid sequence encoding CYP19A variant 1, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:48,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:45 and SEQ ID NO:46,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
13) a probe that specifically hybridizes to a nucleic acid sequence encoding CYP19A variant 2, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:52,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:49 and SEQ ID NO:50,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
14) a probe that specifically hybridizes to a nucleic acid sequence encoding CYP27A1, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:56,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:53 and SEQ ID NO:54,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
15) a probe that specifically hybridizes to a nucleic acid sequence encoding CYP27B1, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:60,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:57 and SEQ ID NO:58,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
16) a probe that specifically hybridizes to a nucleic acid sequence encoding CAR1, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:64,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:61 and SEQ ID NO:62,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
17) a probe that specifically hybridizes to a nucleic acid sequence encoding FXR, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:68,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:65 and SEQ ID NO:66,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
18) a probe that specifically hybridizes to a nucleic acid sequence encoding LXR, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:72,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:69 and SEQ ID NO:70,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
19) a probe that specifically hybridizes to a nucleic acid sequence encoding PPARA, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:76,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:73 and SEQ ID NO:74,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
20) a probe that specifically hybridizes to a nucleic acid sequence encoding PPARD-B, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:80,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:77 and SEQ ID NO:78,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
21) a probe that specifically hybridizes to a nucleic acid sequence encoding PPARG, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:84,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:81 and SEQ ID NO:82,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
22) a probe that specifically hybridizes to a nucleic acid sequence encoding RXRA, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:88,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:85 and SEQ ID NO:86,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
23) a probe that specifically hybridizes to a nucleic acid sequence encoding RXRB, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:92,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:89 and SEQ ID NO:90,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
24) a probe that specifically hybridizes to a nucleic acid sequence encoding RXRG, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:96,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:93 and SEQ ID NO:94,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
25) a probe that specifically hybridizes to a nucleic acid sequence encoding SXR (PXR) transcript variant 1, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:100,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:97 and SEQ ID NO:98,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
26) a probe that specifically hybridizes to a nucleic acid sequence encoding SULT1A1, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:104,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:101 and SEQ ID NO:102,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
27) a probe that specifically hybridizes to a nucleic acid sequence encoding SULT1B1, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:108,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:105 and SEQ ID NO:106,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
28) a probe that specifically hybridizes to a nucleic acid sequence encoding SULT1C1, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:112,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:109 and SEQ ID NO:110,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
29) a probe that specifically hybridizes to a nucleic acid sequence encoding SULT1E1, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:116,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:113 and SEQ ID NO:114,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
30) a probe that specifically hybridizes to a nucleic acid sequence encoding SULT2A1, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:120,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:117 and SEQ ID NO:118,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
31) a probe that specifically hybridizes to a nucleic acid sequence encoding SULT2B1b, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:124,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:121 and SEQ ID NO:122,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
32) a probe that specifically hybridizes to a nucleic acid sequence encoding UGT2A1, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:128,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:125 and SEQ ID NO:126,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
33) a probe that specifically hybridizes to a nucleic acid sequence encoding UGT2B4, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:132,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:129 and SEQ ID NO:130,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
34) a probe that specifically hybridizes to a nucleic acid sequence encoding UGT2B15, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:136,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:133 and SEQ ID NO:134,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
35) a probe that specifically hybridizes to a nucleic acid sequence encoding UGT2B17, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:140,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:137 and SEQ ID NO:138,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
36) a probe that specifically hybridizes to a nucleic acid sequence encoding UGT8, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:144,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:141 and SEQ ID NO:142,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
37) a probe that specifically hybridizes to a nucleic acid sequence encoding CNT1, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:148,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:145 and SEQ ID NO:146,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
38) a probe that specifically hybridizes to a nucleic acid sequence encoding CNT2, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:152,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:149 and SEQ ID NO:150,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
39) a probe that specifically hybridizes to a nucleic acid sequence encoding CNT3, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:156,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:153 and SEQ ID NO:154,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
40) a probe that specifically hybridizes to a nucleic acid sequence encoding ENT1, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:160,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:157 and SEQ ID NO:158,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
41) a probe that specifically hybridizes to a nucleic acid sequence encoding ENT2, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:164,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:161 and SEQ ID NO:162,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
42) a probe that specifically hybridizes to a nucleic acid sequence encoding ENT3, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:168,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:165 and SEQ ID NO:166,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
43) a probe that specifically hybridizes to a nucleic acid sequence encoding LST1, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:172,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:169 and SEQ ID NO:170,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
44) a probe that specifically hybridizes to a nucleic acid sequence encoding LST2, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:176,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:173 and SEQ ID NO:174,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
45) a probe that specifically hybridizes to a nucleic acid sequence encoding LST3, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:180,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:177 and SEQ ID NO:178,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
46) a probe that specifically hybridizes to a nucleic acid sequence encoding NTCP, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:184,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:181 and SEQ ID NO:182,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
47) a probe that specifically hybridizes to a nucleic acid sequence encoding NTCP2, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:188,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:185 and SEQ ID NO:186,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
48) a probe that specifically hybridizes to a nucleic acid sequence encoding OAT1, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:192,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:189 and SEQ ID NO:190,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
49) a probe that specifically hybridizes to a nucleic acid sequence encoding OAT2, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:196,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:193 and SEQ ID NO:194,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
50) a probe that specifically hybridizes to a nucleic acid sequence encoding OAT3, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:200,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:197 and SEQ ID NO:198,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
51) a probe that specifically hybridizes to a nucleic acid sequence encoding OAT4, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:204,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:201 and SEQ ID NO:202,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
52) a probe that specifically hybridizes to a nucleic acid sequence encoding OAT4L, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:208,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:205 and SEQ ID NO:206,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
53) a probe that specifically hybridizes to a nucleic acid sequence encoding OATP-A, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:212,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:209 and SEQ ID NO:210,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
54) a probe that specifically hybridizes to a nucleic acid sequence encoding OATP-B, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:216,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:213 and SEQ ID NO:214,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
55) a probe that specifically hybridizes to a nucleic acid sequence encoding OATP-C, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:220,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:217 and SEQ ID NO:218,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
56) a probe that specifically hybridizes to a nucleic acid sequence encoding OATP-D, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:224,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:221 and SEQ ID NO:222,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
57) a probe that specifically hybridizes to a nucleic acid sequence encoding OATP-E, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:228,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:225 and SEQ ID NO:226,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
58) a probe that specifically hybridizes to a nucleic acid sequence encoding OATP-F, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:232,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:229 and SEQ ID NO:230,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
59) a probe that specifically hybridizes to a nucleic acid sequence encoding OATP-RP1, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:236,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:233 and SEQ ID NO:234,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
60) a probe that specifically hybridizes to a nucleic acid sequence encoding OATP-RP2, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:240,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:237 and SEQ ID NO:238,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
61) a probe that specifically hybridizes to a nucleic acid sequence encoding OATP-RP4, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:244,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:241 and SEQ ID NO:242,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
62) a probe that specifically hybridizes to a nucleic acid sequence encoding OATP-RP5, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:248,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:245 and SEQ ID NO:246,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
63) a probe that specifically hybridizes to a nucleic acid sequence encoding OATP8, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:252,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:249 and SEQ ID NO:250,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
64) a probe that specifically hybridizes to a nucleic acid sequence encoding OCT1, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:256,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:253 and SEQ ID NO:254,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
65) a probe that specifically hybridizes to a nucleic acid sequence encoding OCT2, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:260,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:257 and SEQ ID NO:258,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
66) a probe that specifically hybridizes to a nucleic acid sequence encoding OCTN1, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:264,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:261 and SEQ ID NO:262,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
67) a probe that specifically hybridizes to a nucleic acid sequence encoding OCTN2, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:268,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:265 and SEQ ID NO:266,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
68) a probe that specifically hybridizes to a nucleic acid sequence encoding ORCTL3, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:272,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:269 and SEQ ID NO:270,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
69) a probe that specifically hybridizes to a nucleic acid sequence encoding ORCTL4, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:276,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:273 and SEQ ID NO:274,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
70) a probe that specifically hybridizes to a nucleic acid sequence encoding PGT, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:280,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:277 and SEQ ID NO:278,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
71) a probe that specifically hybridizes to a nucleic acid sequence encoding SLC22A1 L, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:284,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:281 and SEQ ID NO:282,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c);
and
72) a probe that specifically hybridizes to a nucleic acid sequence encoding SLC22A3, wherein the nucleic acid sequence of the probe is selected from the group consisting of:
(a) a nucleic acid sequence consisting of SEQ ID NO:288,
(b) a nucleic acid sequence prepared using amplification and primer pairs having the nucleic acid sequence of SEQ ID NO:285 and SEQ ID NO:286,
(c) a nucleic acid sequence of (a) or (b) wherein T can be U, and
(d) a fragment of (a), (b) or (c).

358 more elements

A method of detecting the expression of two or more genes, comprising the steps:

(a) providing two or more nucleic acid molecules, wherein two of the nucleic acid molecules have a nucleic acid sequence consisting of:
(i) a nucleic acid sequence as shown in SEQ ID NOS:
4, 8, 12, 16, 20, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104, 108, 112, 116, 120, 124, 128, 132, 136, 140, 144, 148, 152, 156, 160, 164, 168, 172, 176, 180, 184, 188, 192, 196, 200, 204, 208, 212, 216, 220, 224, 228, 232, 236, 240, 244, 248, 252, 256, 260, 264, 268, 272, 276, 280, 284, or 288;
(ii) a nucleic acid sequence prepared using amplification and primer pairs, wherein the primer pairs are selected from the following pairs of nucleic acid sequences:
SEQ ID NO:1 and SEQ ID NO:2;
SEQ ID NO:5 and SEQ ID NO:6;
SEQ ID NO:9 and SEQ ID NO:10;
SEQ ID NO:13 and SEQ ID NO:14;
SEQ ID NO:17 and SEQ ID NO:18;
SEQ ID NO:21 and SEQ ID NO:22;
SEQ ID NO:25 and SEQ ID NO:26;
SEQ ID NO:29 and SEQ ID NO:30;
SEQ ID NO:33 and SEQ ID NO:34;
SEQ ID NO:37 and SEQ ID NO:38;
SEQ ID NO:41 and SEQ ID NO:42;
SEQ ID NO:45 and SEQ ID NO:46;
SEQ ID NO:49 and SEQ ID NO:50;
SEQ ID NO:53 and SEQ ID NO:54;
SEQ ID NO:57 and SEQ ID NO:58;
SEQ ID NO:61 and SEQ ID NO:62;
SEQ ID NO:65 and SEQ ID NO:66;
SEQ ID NO:69 and SEQ ID NO:70;
SEQ ID NO:73 and SEQ ID NO:74;
SEQ ID NO:77 and SEQ ID NO:78;
SEQ ID NO:81 and SEQ ID NO:82;
SEQ ID NO:85 and SEQ ID NO:86;
SEQ ID NO:89 and SEQ ID NO:90;
SEQ ID NO:93 and SEQ ID NO:94;
SEQ ID NO:97 and SEQ ID NO:98;
SEQ ID NO:101 and SEQ ID NO:102;
SEQ ID NO:105 and SEQ ID NO:106;
SEQ ID NO:109 and SEQ ID NO:110;
SEQ ID NO:113 and SEQ ID NO:114;
SEQ ID NO:117 and SEQ ID NO:118;
SEQ ID NO:121 and SEQ ID NO:122;
SEQ ID NO:125 and SEQ ID NO:126;
SEQ ID NO:129 and SEQ ID NO:130;
SEQ ID NO:133 and SEQ ID NO:134;
SEQ ID NO:137 and SEQ ID NO:
138;
SEQ ID NO:141 and SEQ ID NO:142;
SEQ ID NO:145 and SEQ ID NO:146;
SEQ ID NO:149 and SEQ ID NO:150;
SEQ ID NO:153 and SEQ ID NO:154;
SEQ ID NO:157 and SEQ ID NO:158;
SEQ ID NO:161 and SEQ ID NO:162;
SEQ ID NO:165 and SEQ ID NO:166;
SEQ ID NO:169 and SEQ ID NO:170;
SEQ ID NO:173 and SEQ ID NO:174;
SEQ ID NO:177 and SEQ ID NO:178;
SEQ ID NO:181 and SEQ ID NO:182;
SEQ ID NO:185 and SEQ ID NO:186;
SEQ ID NO:189 and SEQ ID NO:190;
SEQ ID NO:193 and SEQ ID NO:194;
SEQ ID NO:197 and SEQ ID NO:198;
SEQ ID NO:201 and SEQ ID NO:202;
SEQ ID NO:205 and SEQ ID NO:206;
SEQ ID NO:209 and SEQ ID NO:210;
SEQ ID NO:213 and SEQ ID NO:214;
SEQ ID NO:217 and SEQ ID NO:218;
SEQ ID NO:221 and SEQ ID NO:222;
SEQ ID NO:225 and SEQ ID NO:226;
SEQ ID NO:229 and SEQ ID NO:230;
SEQ ID NO:233 and SEQ ID NO:234;
SEQ ID NO:237 and SEQ ID NO:238;
SEQ ID NO:241 and SEQ ID NO:242;
SEQ ID NO:245 and SEQ ID NO:246;
SEQ ID NO:249 and SEQ ID NO:250;
SEQ ID NO:253 and SEQ ID NO:254;
SEQ ID NO:257 and SEQ ID NO:258;
SEQ ID NO:261 and SEQ ID NO:262;
SEQ ID NO:265 and SEQ ID NO:266;
SEQ ID NO:269 and SEQ ID NO:270;
SEQ ID NO:273 and SEQ ID NO:274;
SEQ ID NO:277 and SEQ ID NO:278;
SEQ ID NO:281 and SEQ ID NO:282;
or
SEQ ID NO:285 and SEQ ID NO:286;
(iii) a nucleic acid sequence in (i) or (ii) wherein T can also be U;
or
(iv) a fragment of (i) to (iii);
(b) providing transcription indicators from a test sample;
(c) allowing the transcription indicators to hybridize with said two or more nucleic acid molecules;
and
(d) detecting hybridization of said transcription indicators with said two or more nucleic acid molecules, wherein hybridization is indicative of the expression of the genes.

82 more elements

A method for screening a compound for its effect on the expression of two or more genes, comprising the steps:

(a) providing a transcription indicator from a test sample from a subject exposed to the compound;
(b) providing two or more nucleic acid molecules, wherein two of the nucleic acid molecules have a nucleic acid sequence consisting of:
(i) a nucleic acid sequence as shown in SEQ ID NOS:
4, 8, 12, 16, 20, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104, 108, 112, 116, 120, 124, 128, 132, 136, 140, 144, 148, 152, 156, 160, 164, 168, 172, 176, 180, 184, 188, 192, 196, 200, 204, 208, 212, 216, 220, 224, 228, 232, 236, 240, 244, 248, 252, 256, 260, 264, 268, 272, 276, 280, 284, or 288;
(ii) a nucleic acid sequence prepared using amplification and primer pairs, wherein the primer pairs are selected from the following pairs of nucleic acid sequences:
SEQ ID NO:1 and SEQ ID NO:2;
SEQ ID NO:5 and SEQ ID NO:6;
SEQ ID NO:9 and SEQ ID NO:10;
SEQ ID NO:13 and SEQ ID NO:14;
SEQ ID NO:17 and SEQ ID NO:18;
SEQ ID NO:21 and SEQ ID NO:22;
SEQ ID NO:25 and SEQ ID NO:26;
SEQ ID NO:29 and SEQ ID NO:30;
SEQ ID NO:33 and SEQ ID NO:34;
SEQ ID NO:37 and SEQ ID NO:38;
SEQ ID NO:41 and SEQ ID NO:42;
SEQ ID NO:45 and SEQ ID NO:46;
SEQ ID NO:49 and SEQ ID NO:50;
SEQ ID NO:53 and SEQ ID NO:54;
SEQ ID NO:57 and SEQ ID NO:58;
SEQ ID NO:61 and SEQ ID NO:62;
SEQ ID NO:65 and SEQ ID NO:66;
SEQ ID NO:69 and SEQ ID NO:70;
SEQ ID NO:73 and SEQ ID NO:74;
SEQ ID NO:77 and SEQ ID NO:78;
SEQ ID NO:81 and SEQ ID NO:82;
SEQ ID NO:85 and SEQ ID NO:86;
SEQ ID NO:89 and SEQ ID NO:90;
SEQ ID NO:93 and SEQ ID NO:94;
SEQ ID NO:97 and SEQ ID NO:98;
SEQ ID NO:101 and SEQ ID NO:102;
SEQ ID NO:105 and SEQ ID NO:106;
SEQ ID NO:109 and SEQ ID NO:110;
SEQ ID NO:113 and SEQ ID NO:114;
SEQ ID NO:117 and SEQ ID NO:118;
SEQ ID NO:121 and SEQ ID NO:122;
SEQ ID NO:125 and SEQ ID NO:126;
SEQ ID NO:129 and SEQ ID NO:130;
SEQ ID NO:133 and SEQ ID NO:134;
SEQ ID NO:137 and SEQ ID NO:
138;
SEQ ID NO:141 and SEQ ID NO:142;
SEQ ID NO:145 and SEQ ID NO:146;
SEQ ID NO:149 and SEQ ID NO:150;
SEQ ID NO:153 and SEQ ID NO:154;
SEQ ID NO:157 and SEQ ID NO:158;
SEQ ID NO:161 and SEQ ID NO:162;
SEQ ID NO:165 and SEQ ID NO:166;
SEQ ID NO:169 and SEQ ID NO:170;
SEQ ID NO:173 and SEQ ID NO:174;
SEQ ID NO:177 and SEQ ID NO:178;
SEQ ID NO:181 and SEQ ID NO:182;
SEQ ID NO:185 and SEQ ID NO:186;
SEQ ID NO:189 and SEQ ID NO:190;
SEQ ID NO:193 and SEQ ID NO:194;
SEQ ID NO:197 and SEQ ID NO:198;
SEQ ID NO:201 and SEQ ID NO:202;
SEQ ID NO:205 and SEQ ID NO:206;
SEQ ID NO:209 and SEQ ID NO:210;
SEQ ID NO:213 and SEQ ID NO:214;
SEQ ID NO:217 and SEQ ID NO:218;
SEQ ID NO:221 and SEQ ID NO:222;
SEQ ID NO:225 and SEQ ID NO:226;
SEQ ID NO:229 and SEQ ID NO:230;
SEQ ID NO:233 and SEQ ID NO:234;
SEQ ID NO:237 and SEQ ID NO:238;
SEQ ID NO:241 and SEQ ID NO:242;
SEQ ID NO:245 and SEQ ID NO:246;
SEQ ID NO:249 and SEQ ID NO:250;
SEQ ID NO:253 and SEQ ID NO:254;
SEQ ID NO:257 and SEQ ID NO:258;
SEQ ID NO:261 and SEQ ID NO:262;
SEQ ID NO:265 and SEQ ID NO:266;
SEQ ID NO:269 and SEQ ID NO:270;
SEQ ID NO:273 and SEQ ID NO:274;
SEQ ID NO:277 and SEQ ID NO:278;
SEQ ID NO:281 and SEQ ID NO:282;
or
SEQ ID NO:285 and SEQ ID NO:286;
(iii) a nucleic acid sequence in (i) or (ii) wherein T can also be U;
or
(iv) a fragment of (i) to (iii);
(c) allowing said transcription indicator to hybridize with said two or more nucleic acid molecules;
and
(d) detecting hybridization of said transcription indicator with said two or more nucleic acid molecules, wherein hybridization is indicative of the expression of the two or more genes.

82 more elements

An isolated nucleic acid molecule having a nucleic acid sequence consisting of:

(a) a nucleic acid sequence as shown in SEQ ID NOS:
4, 8, 12, 16, 20, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104, 108, 112, 116, 120, 124, 128, 132, 136, 140, 144, 148, 152, 156, 160, 164, 168, 172, 176, 180, 184, 188, 192, 196, 200, 204, 208, 212, 216, 220, 224, 228, 232, 236, 240, 244, 248, 252, 256, 260, 264, 268, 272, 276, 280, 284, or 288;
(b) a nucleic acid sequence prepared using amplification and primer pairs, wherein the primer pairs are selected from the following pairs of nucleic acid sequences:
SEQ ID NO:1 and SEQ ID NO:2;
SEQ ID NO:5 and SEQ ID NO:6;
SEQ ID NO:9 and SEQ ID NO:10;
SEQ ID NO:13 and SEQ ID NO:14;
SEQ ID NO:17 and SEQ ID NO:18;
SEQ ID NO:21 and SEQ ID NO:22;
SEQ ID NO:25 and SEQ ID NO:26;
SEQ ID NO:29 and SEQ ID NO:30;
SEQ ID NO:33 and SEQ ID NO:34;
SEQ ID NO:37 and SEQ ID NO:38;
SEQ ID NO:41 and SEQ ID NO:42;
SEQ ID NO:45 and SEQ ID NO:46;
SEQ ID NO:49 and SEQ ID NO:50;
SEQ ID NO:53 and SEQ ID NO:54;
SEQ ID NO:57 and SEQ ID NO:58;
SEQ ID NO:61 and SEQ ID NO:62;
SEQ ID NO:65 and SEQ ID NO:66;
SEQ ID NO:69 and SEQ ID NO:70;
SEQ ID NO:73 and SEQ ID NO:74;
SEQ ID NO:77 and SEQ ID NO:78;
SEQ ID NO:81 and SEQ ID NO:82;
SEQ ID NO:85 and SEQ ID NO:86;
SEQ ID NO:89 and SEQ ID NO:90;
SEQ ID NO:93 and SEQ ID NO:94;
SEQ ID NO:97 and SEQ ID NO:98;
SEQ ID NO:101 and SEQ ID NO:102;
SEQ ID NO:105 and SEQ ID NO:106;
SEQ ID NO:109 and SEQ ID NO:110;
SEQ ID NO:113 and SEQ ID NO:114;
SEQ ID NO:117 and SEQ ID NO:118;
SEQ ID NO:121 and SEQ ID NO:122;
SEQ ID NO:125 and SEQ ID NO:126;
SEQ ID NO:129 and SEQ ID NO:130;
SEQ ID NO:133 and SEQ ID NO:134;
SEQ ID NO:137 and SEQ ID NO:
138;
SEQ ID NO:141 and SEQ ID NO:142;
SEQ ID NO:145 and SEQ ID NO:146;
SEQ ID NO:149 and SEQ ID NO:150;
SEQ ID NO:153 and SEQ ID NO:154;
SEQ ID NO:157 and SEQ ID NO:158;
SEQ ID NO:161 and SEQ ID NO:162;
SEQ ID NO:165 and SEQ ID NO:166;
SEQ ID NO:169 and SEQ ID NO:170;
SEQ ID NO:173 and SEQ ID NO:174;
SEQ ID NO:177 and SEQ ID NO:178;
SEQ ID NO:181 and SEQ ID NO:182;
SEQ ID NO:185 and SEQ ID NO:186;
SEQ ID NO:189 and SEQ ID NO:190;
SEQ ID NO:193 and SEQ ID NO:194;
SEQ ID NO:197 and SEQ ID NO:198;
SEQ ID NO:201 and SEQ ID NO:202;
SEQ ID NO:205 and SEQ ID NO:206;
SEQ ID NO:209 and SEQ ID NO:210;
SEQ ID NO:213 and SEQ ID NO:214;
SEQ ID NO:217 and SEQ ID NO:218;
SEQ ID NO:221 and SEQ ID NO:222;
SEQ ID NO:225 and SEQ ID NO:226;
SEQ ID NO:229 and SEQ ID NO:230;
SEQ ID NO:233 and SEQ ID NO:234;
SEQ ID NO:237 and SEQ ID NO:238;
SEQ ID NO:241 and SEQ ID NO:242;
SEQ ID NO:245 and SEQ ID NO:246;
SEQ ID NO:249 and SEQ ID NO:250;
SEQ ID NO:253 and SEQ ID NO:254;
SEQ ID NO:257 and SEQ ID NO:258;
SEQ ID NO:261 and SEQ ID NO:262;
SEQ ID NO:265 and SEQ ID NO:266;
SEQ ID NO:269 and SEQ ID NO:270;
SEQ ID NO:273 and SEQ ID NO:274;
SEQ ID NO:277 and SEQ ID NO:278;
SEQ ID NO:281 and SEQ ID NO:282;
or
SEQ ID NO:285 and SEQ ID NO:286;
(c) a nucleic acid sequence in (a) or (b) wherein T can also be U;
or
(d) a fragment of (a) to (c).

77 more elements

Dependencies are inferred from the claim text.

Facts

Application: 12/513,962Filed 8 Nov 2007
Priority: 10 Nov 2006
Published: 2 Dec 2010
Term ends: Not estimatedThe source records don’t support an estimate.

Classification6 codes

C12Q 1/6883
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes: for diseases caused by alterations of genetic material
C12N 9/0071
Oxidoreductases: acting on paired donors with incorporation of molecular oxygen
C12Q 2600/136
Oligonucleotides characterized by their use: screening for pharmacological compounds
C12Q 2600/158
Oligonucleotides characterized by their use: expression markers

Family

Application60/865,266Us provisional application · filed 10 Nov 2006
US 2010/0304984 A1This patent

From the US filing history. Foreign family members aren’t listed.

Timeline

Citations

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Abstract

An array comprising two or more nucleic acid molecules immobilized on a substrate, wherein at least two of the nucleic acid molecules have a nucleic acid sequence consisting of:

(a) a nucleic acid sequence as shown in SEQ ID NOS:
4, 8, 12, 16, 20, 24, 28, 32, 36, 40, 44, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104, 108, 112, 116, 120, 124, 128, 132, 136, 140, 144, 148, 152, 156, 160, 164, 168, 172, 176, 180, 184, 188, 192, 196, 200, 204, 208, 212, 216, 220, 224, 228, 232, 236, 240, 244, 248, 252, 256, 260, 264, 268, 272, 276, 280, 284, or 288;
(b) a nucleic acid sequence prepared using amplification and primer pairs, wherein the primer pairs are selected from the following pairs of nucleic acid sequences:
SEQ ID NO:1 and SEQ ID NO:2;
SEQ ID NO:5 and SEQ ID NO:6;
SEQ ID NO:9 and SEQ ID NO:10;
SEQ ID NO:13 and SEQ ID NO:14;
SEQ ID NO:17 and SEQ ID NO:18;
SEQ ID NO:21 and SEQ ID NO:22;
SEQ ID NO:25 and SEQ ID NO:26;
SEQ ID NO:29 and SEQ ID NO:30;
SEQ ID NO:33 and SEQ ID NO:34;
SEQ ID NO:37 and SEQ ID NO:38;
SEQ ID NO:41 and SEQ ID NO:42;
SEQ ID NO:45 and SEQ ID NO:46;
SEQ ID NO:49 and SEQ ID NO:50;
SEQ ID NO:53 and SEQ ID NO:54;
SEQ ID NO:57 and SEQ ID NO:58;
SEQ ID NO:61 and SEQ ID NO:62;
SEQ ID NO:65 and SEQ ID NO:66;
SEQ ID NO:69 and SEQ ID NO:70;
SEQ ID NO:73 and SEQ ID NO:74;
SEQ ID NO:77 and SEQ ID NO:78;
SEQ ID NO:81 and SEQ ID NO:82;
SEQ ID NO:85 and SEQ ID NO:86;
SEQ ID NO:89 and SEQ ID NO:90;
SEQ ID NO:93 and SEQ ID NO:94;
SEQ ID NO:97 and SEQ ID NO:98;
SEQ ID NO:101 and SEQ ID NO:102;
SEQ ID NO:105 and SEQ ID NO:106;
SEQ ID NO:109 and SEQ ID NO:110;
SEQ ID NO:113 and SEQ ID NO:114;
SEQ ID NO:117 and SEQ ID NO:118;
SEQ ID NO:121 and SEQ ID NO:122;
SEQ ID NO:125 and SEQ ID NO:126;
SEQ ID NO:129 and SEQ ID NO:130;
SEQ ID NO:133 and SEQ ID NO:134;
SEQ ID NO:137 and SEQ ID NO:
138;
SEQ ID NO:141 and SEQ ID NO:142;
SEQ ID NO:145 and SEQ ID NO:146;
SEQ ID NO:149 and SEQ ID NO:150;
SEQ ID NO:153 and SEQ ID NO:154;
SEQ ID NO:157 and SEQ ID NO:158;
SEQ ID NO:161 and SEQ ID NO:162;
SEQ ID NO:165 and SEQ ID NO:166;
SEQ ID NO:169 and SEQ ID NO:170;
SEQ ID NO:173 and SEQ ID NO:174;
SEQ ID NO:177 and SEQ ID NO:178;
SEQ ID NO:181 and SEQ ID NO:182;
SEQ ID NO:185 and SEQ ID NO:186;
SEQ ID NO:189 and SEQ ID NO:190;
SEQ ID NO:193 and SEQ ID NO:194;
SEQ ID NO:197 and SEQ ID NO:198;
SEQ ID NO:201 and SEQ ID NO:202;
SEQ ID NO:205 and SEQ ID NO:206;
SEQ ID NO:209 and SEQ ID NO:210;
SEQ ID NO:213 and SEQ ID NO:214;
SEQ ID NO:217 and SEQ ID NO:218;
SEQ ID NO:221 and SEQ ID NO:222;
SEQ ID NO:225 and SEQ ID NO:226;
SEQ ID NO:229 and SEQ ID NO:230;
SEQ ID NO:233 and SEQ ID NO:234;
SEQ ID NO:237 and SEQ ID NO:238;
SEQ ID NO:241 and SEQ ID NO:242;
SEQ ID NO:245 and SEQ ID NO:246;
SEQ ID NO:249 and SEQ ID NO:250;
SEQ ID NO:253 and SEQ ID NO:254;
SEQ ID NO:257 and SEQ ID NO:258;
SEQ ID NO:261 and SEQ ID NO:262;
SEQ ID NO:265 and SEQ ID NO:266;
SEQ ID NO:269 and SEQ ID NO:270;
SEQ ID NO:273 and SEQ ID NO:274;
SEQ ID NO:277 and SEQ ID NO:278;
SEQ ID NO:281 and SEQ ID NO:282;
or
SEQ ID NO:285 and SEQ ID NO:286;
(c) a nucleic acid sequence in (a) or (b) wherein T can also be U;
or
(d) a fragment of (a) to (c).

77 more elements

Facts

Application

12/513,962Filed 8 Nov 2007

Priority

10 Nov 2006

Published

2 Dec 2010

Term ends

Not estimatedThe source records don’t support an estimate.

Classification6 codes

C12Q 1/6883
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes: for diseases caused by alterations of genetic material
C12N 9/0071
Oxidoreductases: acting on paired donors with incorporation of molecular oxygen
C12Q 2600/136
Oligonucleotides characterized by their use: screening for pharmacological compounds
C12Q 2600/158
Oligonucleotides characterized by their use: expression markers