Nano-biosensor for biomolecular recognition and a method of synthesizing the same

A method of synthesizing a nano-biosensor for bimolecular recognition consisting the steps of:

• preparing a micro-emulsion solution by mixing cyclohexane, Triton X-100 and n-hexanol;
• transferring the micro-emulsion solution into a first beaker and a second beaker wherein the amount of the micro-emulsion solution in the first beaker and the amount of the micro-emulsion solution in the second beaker are equal;
• preparing an aqueous stock solution of ZnSO4.7H2O with a morality of 0.1;
• preparing a pluralities of aqueous stock solutions of Mn(NO3)2.4H2O with different concentrations and wherein the different concentrations are 0.01%, 0.7%, 2%, 3%, 5.5%, 9% and 13%;
• preparing an aqueous stock solution of Na2S with a morality of 0.1;
• adding the aqueous stock solution of ZnSO4.7H2O and the pluralities of aqueous stock solutions of Mn(NO3)2.4H2O with different concentrations to the first beaker;
• agitating a content of the first beaker after adding the aqueous stock solution of ZnSO4.7H2O and the pluralities of aqueous stock solutions of Mn(NO3)2.4H2O with different concentrations;
• adding the aqueous stock solution of Na2S to the second beaker;
• agitating a content of the second beaker after adding the aqueous stock solution of Na2S;
• mixing the content of the first beaker and the content of the second beaker to obtain a resultant mixture;
• agitating the resultant mixture;
• keeping the resultant mixture at room temperature for aging;
• adding a linking agent to the resultant mixture after aging;
• and
• adding a biological agent to the resultant mixture to obtain a nano particle of ZnS:Mn.