Selective enrichment of post-translationally modified proteins and/or peptides from complex samples
AssigneeKoninklijke Philips Electronics N.V.
InventorsRalf Hoffmann, Hugo M. Visser, Edwin P. Romijn
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Abstract
The present invention relates to the selective enrichment of post-translationally modified proteins and/or peptides from complex samples by combining a particular protein/peptide labeling and fractionation strategy with specific chemical and/or enzymatic reactions targeting the post-translational modification to be analyzed. More specifically, the invention relates to methods for the enrichment and/or separation of phospho-proteins and/or -peptides, and particularly for the discrimination between different subsets of phospho-proteins and/or -peptides in complex samples.
Claims14
Method for the selective enrichment and/or separation of phospho-proteins and/or -peptides in a sample, comprising:
- (a) single or double chemical labeling of the proteins and/or peptides comprised in the sample
- (b) fractionating the proteins and/or peptides;
- (c) removing or altering the phosphate-group from at least a first subset of phospho-proteins and/or -peptides;
- (d) re-fractionating the proteins and/or peptides;
- (e) comparing the fractionation patterns obtained in steps (b) and (d);
- and
- (f) separating the at least first subset of phospho-proteins and/or -peptides modified in step (c) based on the results obtained in step (e).
Dependencies are inferred from the claim text.