Soft tissue processing

A method for the treatment of soft fascia tissue to prepare the same for implantation into a human comprising the steps of:

• (a) thawing frozen donor fascia tissue;
• (b) inspecting the donor tissue for defects;
• (c) trimming the donor tissue for processing;
• (d) soaking the trimmed donor tissue in a compound antibiotic mixture from 1.5 to 24 hours;
• (e) rinsing the antibiotic mixture from the tissue with sterile water;
• (f) cutting the treated tissue to a specific size;
• (g) perforating the cut tissue with a plurality of spaced apertures;
• (h) immersing the perforated cut tissue in ethanol;
• and (i) packaging the ethanol soaked perforated tissue in a sealed package.

A method for the treatment of cartilage to prepare the same for implantation into a human comprising the steps of:

• (a) thawing frozen cartilage tissue;
• (b) soaking the donor tissue in a compound antibiotic mixture from 1.5 to 24 hours;
• (c) rinsing the antibiotic mixture from the tissue with phosphate buffered saline;
• (d) lyophilizing the treated tissue to achieve a residential moisture level between 0.1% to 8.0%;
• and (e) milling the cartilage pieces to sizes less than 1 mm.

A method of preparing soft tissue suitable for implantation into a human, comprising:

• (a) decellularizing donor soft tissue;
• (b) treating donor soft tissue with a detergent for a period of time ranging from about 1.5 to 48 hours;
• (c) treating donor soft tissue in a sterilization solution, wherein the sterilization solution comprises peracetic acid, ethanol, propylene glycol, and water;
• and
• (d) packaging donor soft tissue in a sealed package.