Methods of generating embryoid bodies and uses of same
AssigneeTechnion Resezrch & Development Foundation Ltd.
InventorsGalia Yirme, Joseph Itskovitz-Eldor
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Abstract
Methods of generating embryoid bodies (EBs) by culturing embryonic stem cells (ESCs) under static conditions followed by culturing the cells under dynamic conditions using e.g., a Glass Bulb-shaped Impeller (GBI) or shaking a culture vessel are provided. Also provided are methods of generating expanded and/or differentiated cells from the EBs of the invention and methods of using same for treating disorders requiring cell replacement therapy.
Claims25
A method of generating embryoid bodies, comprising:
- (a) culturing embryonic stem cells under static conditions;
- and subsequently
- (b) culturing said embryonic stem cells under dynamic conditions using a Glass Bulb-shaped Impeller (GBI);
- thereby generating the embryoid bodies.
A method of generating embryoid bodies, comprising:
- (a) culturing embryonic stem cells under static conditions;
- and subsequently
- (b) culturing said embryonic stem cells under dynamic conditions which comprise shaking a culture vessel comprising said embryonic stem cells;
- thereby generating the embryoid bodies.
A method of generating embryoid bodies, comprising:
- (a) culturing embryonic stem cells under static conditions;
- and subsequently
- (b) culturing said embryonic stem cells under dynamic conditions so as to obtain at least 5 fold expansion following six days of said culturing under said dynamic conditions;
- thereby generating the embryoid bodies.
A method of generating embryoid bodies, comprising:
- (a) culturing embryonic stem cells under static conditions;
- and subsequently
- (b) culturing said embryonic stem cells under dynamic conditions so as to obtain embryoid bodies which include no more than 4% of apoptotic cells following 10 days in culture;
- thereby generating the embryoid bodies.
Dependencies are inferred from the claim text.