Cell rolling separation
AssigneeMassachusetts Institute of Technology
InventorsRohit Nandkumar KARNIK, Seungpyo HONG, Ying MEI, Daniel Griffith ANDERSON, Jeffrey Michael KARP, Robert S. LANGER, Suman BOSE
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Abstract
The present invention provides systems for cell separation based on cell rolling on surfaces along edges of regions coated with cell adhesion molecules. A variety of designs of coated regions and edges are disclosed.
Claims58
A method comprising steps of:
- providing a surface that is at least partially coated with an ordered layer of cell adhesion molecules, wherein the surface comprises at least one edge between an area coated with the ordered layer and another area that is not coated with the ordered layer;
- and
- flowing a population of cells across the surface in a direction which forms a non-zero angle αs with the at least one edge, wherein at least one cell in the population of cells comprises a surface moiety that is recognized by the cell adhesion molecules and wherein the at least one cell rolls for a period of time in a direction that is αs to the direction of flow as a result of interacting with at least a portion of the at least one edge.
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The method of claim 29, wherein the at least one cell has an average diameter d and wherein ws<3d.
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A device comprising:
- a separation flow chamber,
- wherein the separation flow chamber comprises a surface that is at least partially coated with an ordered layer of cell adhesion molecules, and the surface comprises at least one edge between an area coated with the ordered layer and another area that is not coated with the ordered layer;
- an inlet for flowing cells into the separation flow chamber;
- and
- an outlet for flowing cells out of the separation flow chamber;
- wherein when cells are flowed through the inlet to the outlet they flow at an angle αs to the direction of the at least one edge unless they interact with the at least one edge.
125-155. (canceled)
A method comprising steps of:
- providing a three dimensional surface that is at least partially coated with an ordered layer of cell adhesion molecules, and
- flowing a population of cells across the surface in such conditions to create a stagnation line of no flow, wherein the direction of flow forms a non-zero angle αs with the stagnation line, wherein at least one cell in the population of cells comprises a surface moiety that is recognized by the cell adhesion molecules and wherein at least one cell in the population of cells rolls at least part of the time in a direction that is αs to the direction of flow.
157-158. (canceled)
Dependencies are inferred from the claim text.