T-cell vaccination in the treatment of hiv infection
AssigneesYeda Research and Development Co. Ltd., Hadasit Medical Research Services and Development Ltd.
InventorsRivka Abulafia-Lapid, Henri Atlan, Irun R. Cohen
Life of this patent
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Abstract
A method for the preparation of a T cell vaccine for the treatment of immunodeficient HIV-infected patients is described herein, based on the enrichment of autologous CD4-reactive CD8 T cells. Also described is a protocol for the implementation of T cell vaccination in immunodeficient HIV-infected, as well as a method of treatment, based on the T cell vaccine developed herein. Finally, kits for preparing the T cell vaccine as well as for implementing the protocol are also provided.
Claims3
1-6. (canceled)
A kit for preparing a T cell vaccine against anti-CD4 autoimmune T cells, wherein said vaccine is to be used as therapy in subjects suffering from HIV infection-induced immunodeficiency, and wherein said kit comprises:
- a) means for obtaining peripheral blood mononuclear cells (PBMC);
- b) means for cultivating PBMC;
- c) stimulating factor, preferably PHA;
- d) IL-2;
- e) irradiated CD4-pulsed antigen presenting cells;
- f) a fixating agent, preferably glutaraldehyde;
- g) a neutralizing agent, preferably glycine;
- h) injection men-grade saline;
- i) manual with instructions on how to prepare the T cell vaccine.
A kit for implementing a protocol for T cell vaccination of immunodeficient HIV-infected patients, said kit comprising the tools necessary for preparing a T cell vaccine against anti-CD4 autoimmune T cells, as follows:
- a) means for obtaining peripheral blood mononuclear cells (PBMC);
- b) means for cultivating PMBC;
- c) stimulating factor, preferably PHA;
- d) IL-2;
- e) irradiated CD4-pulsed antigen presenting cells;
- f) a fixating agent, preferably glutaraldehyde;
- g) a neutralizing agent, preferably glycine;
- h) injection Injection grade saline;
- g) manual with instructions on how to prepare the T cell vaccine;
- and further comprising instructions for implementing said protocol.
Dependencies are inferred from the claim text.