Swellable synthetic microcarriers for culturing cells
AssigneeCorning Incorporated
InventorsSophie Deshayes, David Henry, Martial Hervy
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Abstract
A cell culture microcarrier includes a polymer formed from copolymerization of a mixture including (i) an uncharged hydrophilic unsaturated monomer having a hydroxyl group; (ii) a hydrophilic carboxylic acid containing unsaturated monomer; and (iii) a hydrophilic multifunctional unsaturated monomer. The microcarrier may further include a polypeptide, such as a polypeptide that promotes cell adhesion, conjugated to the surface of the microcarrier; e.g. via the carboxyl group from the hydrophilic carboxylic acid containing unsaturated monomer. Some of the microcarriers support attachment of human embryonic stem cells.
Claims27
A microcarrier for cell culture, comprising:
- a polymeric microcarrier base formed from copolymerization of a mixture of monomers including
- (i) an uncharged hydrophilic unsaturated monomer having a hydroxyl group selected from a hydrophilic (meth)acrylate monomer having a hydroxyl group or a hydrophilic (meth)acrylamide monomer having a hydroxyl group,
- (ii) a hydrophilic carboxylic acid containing unsaturated monomer selected from a carboxylic acid containing (meth)acrylate monomer or a carboxylic acid containing (meth)acrylamide monomer, and
- (iii) a first hydrophilic multifunctional unsaturated monomer selected from a hydrophilic multifunctional (meth)acrylate monomer or a hydrophilic multifunctional (meth)acrylamide monomer;
- and
- a polypeptide conjugated to the microcarrier base,
- wherein the microcarrier base has an equilibrium water content of greater than 75%.
Dependencies are inferred from the claim text.