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US 2010/0304471 A1Published application
Assay
OwnerJun Tao, Helen Grace Huanginventors
Life of this patent
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Abstract
The present invention relates to test devices, and in particular devices capable of detecting the presence or absence of an analyte in a sample, such as a liquid sample. Also provided are methods of using such devices for quantitative or qualitative measurement of one or more analytes in a liquid sample.
Claims25
A test device for detecting an analyte in a liquid sample, comprising;
- a matrix for supporting the liquid sample flowing thereon comprising:
- (a) a blocking zone comprising a blocking-zone binding agent immobilized thereon, wherein said analyte in said liquid sample and a conjugate comprising an analyte mimic when present, compete for binding to said blocking-zone binding agent, wherein binding affinity between the analyte mimic and the blocking-zone binding agent is higher than that between the analyte and the blocking-zone binding agent;
- (b) a detection zone comprising immobilized thereon a detection-zone binding agent that exhibits binding specificity to the conjugate comprising said analyte mimic.
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A test device for detecting an analyte in a liquid sample, comprising;
- a matrix for supporting the liquid sample flowing thereon comprising:
- (a) a blocking zone comprising a blocking-zone binding agent, wherein the blocking-zone binding agent comprises an analyte mimic immobilized on the blocking zone, wherein said analyte in said liquid sample and said analyte mimic compete for binding to a conjugate when present, wherein binding affinity between the analyte mimic and the conjugate is higher than that between the analyte and the conjugate;
- (b) a detection zone comprising, immobilized thereon, a detection-zone binding agent that exhibits binding specificity to said conjugate.
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Dependencies are inferred from the claim text.