Method for introducing nucleic acids into fungal cells
AssigneeDanisco US Inc., Genencor Division
InventorsSteven Kim, Andrei Miasnikov
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Abstract
The method disclosed herein, relates generally to introducing molecules such as biomolecules (e.g., nucleic acids) into a filamentous fungus. More specifically, the methods disclosed herein relate to introducing one or more nucleic acids into a filamentous fungus.
Claims20
A method for introducing one or more nucleic acids into filamentous fungal spores to produce transformed fungal spores, comprising
- mixing one or more filamentous fungal spores with one or more nucleic acids;
- and
- applying an electric field under conditions that allow introduction of the one or more nucleic acids into one or more fungal spores, wherein the electric field is applied at a voltage of between about 11,000 V/cm and about 20,000 V/cm to produce filamentous fungal spores.
A method for introducing one or more nucleic acids into a population of filamentous fungal spores, comprising
- contacting a population of filamentous fungal spores with one or more nucleic acids to produce transformed fungal spores;
- and
- applying an electric field under conditions that allow introduction of the nucleic acids into the fungal spores, wherein the electric field is applied at a voltage of between about 15,500 V/cm and about 18,500 V/cm in the absence of lithium acetate, to produce transformed fungal spores.
A method for introducing one or more nucleic acids into a population of Trichoderma spores to provide transformed Trichoderma spores, comprising
- contacting a population of Trichoderma spores with one or more nucleic acids;
- and
- applying an electric field of between 15,500 V/cm and about 18,500 V/cm under conditions that allow introduction of the one or more nucleic acids into the fungal spores to produce transformed Trichoderma fungal spores.
Dependencies are inferred from the claim text.