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US 2010/0304467 A1Published application
Methods for producing human ceramide using yeast transformants
AssigneesSuntory Holdings Limited, Hiroshima University
InventorsYukiko Kodama, Hiroaki Okuhara, Koichi Funato
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Abstract
The present invention provides a method for producing human ceramide in a yeast cell. The method of the present invention comprises: 1) introducing the sphingoid Δ4-desaturase gene (DES1) by transformation of the yeast cell; 2) abolishing the expression of the yeast sphinganine C4-hydroxylase gene (SUR2) by transformation of the yeast cell; and 3) abolishing the expression of the yeast sphingoid base kinase gene (LCB4) by transformation of the yeast cell.
Claims8
A method for producing human ceramide in a yeast cell, which comprises:
- 1) introducing the sphingoid M-desaturase gene (DES1) by transformation of the yeast cell;
- 2) abolishing the expression of the yeast sphinganine C4-hydroxylase gene (SUR2) by transformation of the yeast cell;
- and
- 3) abolishing the expression of the yeast sphingoid base kinase gene (LCB4) by transformation of the yeast cell.
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Dependencies are inferred from the claim text.