Glucose dehydrogenase polypeptides and related polynucleotides
AssigneeCodexis, Inc.
InventorsS. Christopher Davis, Stephane J. Jenne, Anke Krebber, Lisa Marie Newman
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Abstract
The present invention is directed to glucose dehydrogenase (GDH) polypeptides that have enhanced GDH activity and/or thermostability relative to the backbone wild-type glucose dehydrogenase polypeptide. In addition, the present invention is directed to a polynucleotide that encodes for the GDH polypeptides of the present invention, to nucleic acid sequences comprising the polynucleotides, to expression vectors comprising the polynucleotides operatively linked to a promoter, to host cells transformed to express the GDH polypeptides, and to a method for producing the GDH polypeptides of the present invention.
Claims21
A polynucleotide comprising a nucleic acid sequence encoding a non-naturally occurring glucose dehydrogenase (GDH) polypeptide capable of converting glucose and nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP) to gluconolactone and reduced NADH or reduced NADPH with at least 1.5 times the initial glucose dehydrogenase (GDH) activity of the wild-type GDH of SEQ ID NO:
- 2 and which polypeptide comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:54, 74, or 84.
Dependencies are inferred from the claim text.