Microorganisms and methods for conversion of syngas and other carbon sources to useful products

(canceled)

4-6. (canceled)

8-17. (canceled)

(canceled)

24-28. (canceled)

A non-naturally occurring microbial organism, comprising a microbial organism having a 4-hydroxybutryate pathway comprising at least one exogenous nucleic acid encoding an 4-hydroxybutryate pathway enzyme expressed in a sufficient amount to produce 4-hydroxybutryate, said 4-hydroxybutryate pathway enzyme comprising an acetoacetyl-CoA thiolase, a 3-hydroxybutyryl-CoA dehydrogenase, a crotonase, a crotonyl-CoA hydratase, a 4-hydroxybutyryl-CoA transferase, a 4-hydroxybutyryl-CoA hydrolase, a 4-hydroxybutyryl-CoA synthetase, a phosphotrans-4-hydroxybutyrylase, and a 4-hydroxybutyrate kinase.

31-37. (canceled)

39-48. (canceled)

(canceled)

55-59. (canceled)

A non-naturally occurring microbial organism comprising a microbial organism having a 1,4-butanediol pathway comprising at least one exogenous nucleic acid encoding a 1,4-butanediol pathway enzyme expressed in a sufficient amount to produce 1,4-butanediol, said 1,4-butanediol pathway enzyme comprising an acetoacetyl-CoA thiolase, a 3-Hydroxybutyryl-CoA dehydrogenase, a crotonase, a crotonyl-CoA hydratase, a 4-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl-CoA reductase (aldehyde forming), and a 1,4-butanediol dehydrogenase;

• said organism further comprising an acetyl-CoA pathway comprising at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme expressed in a sufficient amount to produce acetyl-CoA, said acetyl-CoA pathway enzyme comprising a corrinoid protein, a methyltetrahydrofolate:corrinoid protein methyltransferase, a corrinoid iron-sulfur protein, a nickel-protein assembly protein, a ferredoxin, an acetyl-CoA synthase, a carbon monoxide dehydrogenase, a pyruvate ferredoxin oxidoreductase, and a hydrogenase.

62-78. (canceled)

(canceled)

A non-naturally occurring microbial organism comprising a microbial organism having a 1,4-butanediol pathway comprising at least one exogenous nucleic acid encoding a 1,4-butanediol pathway enzyme expressed in a sufficient amount to produce 1,4-butanediol, said 1,4-butanediol pathway enzyme comprising an acetoacetyl-CoA thiolase, a 3-Hydroxybutyryl-CoA dehydrogenase, a crotonase, a crotonyl-CoA hydratase, a 4-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl-CoA reductase (aldehyde forming), and a 1,4-butanediol dehydrogenase;

• said organism further comprising an acetyl-CoA pathway comprising at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme expressed in a sufficient amount to produce acetyl-CoA, said acetyl-CoA pathway enzyme comprising an acetyl-CoA synthase, a formate dehydrogenase, a formyltetrahydrofolate synthetase, a methenyltetrahydrofolate cyclohydrolase, a methylenetetrahydrofolate dehydrogenase, and a methylenetetrahydrofolate reductase.

85-89. (canceled)

A non-naturally occurring microbial organism comprising a microbial organism having an isopropanol pathway comprising at least one exogenous nucleic acid encoding an isopropanol pathway enzyme expressed in a sufficient amount to produce isopropanol, said isopropanol pathway enzyme comprising an acetoacetyl-CoA thiolase, an acetoacetyl-CoA transferase, an acetoacetyl-CoA hydrolase, an acetoacetyl-CoA synthetase, a phosphotransacetoacetylase, an acetoacetate kinase, an acetoacetate decarboxylase, and an isopropanol dehydrogenase;

• said organism further comprising at least one exogenous nucleic acid encoding an acetyl-CoA enzyme expressed in a sufficient amount to produce acetyl-CoA, said acetyl-CoA pathway enzyme comprising a methanol methyl transferase, a corrinoid protein, a methyltetrahydro-folate:corrinoid protein methyltransferase, a corrinoid iron-sulfur protein, a nickel-protein assembly protein, a ferredoxin, an acetyl-CoA synthase, a carbon monoxide dehydrogenase, a pyruvate ferredoxin oxidoreductase, and a hydrogenase.

(canceled)

A non-naturally occurring microbial organism, comprising a microbial organism having an isopropanol pathway comprising at least one exogenous nucleic acid encoding an isopropanol pathway enzyme expressed in a sufficient amount to produce isopropanol, said isopropanol pathway enzyme comprising an acetoacetyl-CoA thiolase, an acetoacetyl-CoA transferase, an acetoacetyl-CoA hydrolase, an acetoacetyl-CoA synthetase, a phosphotransacetoacetylase, an acetoacetate kinase, an acetoacetate decarboxylase, and an isopropanol dehydrogenase;

• said organism further comprising at least one exogenous nucleic acid encoding an acetyl-CoA enzyme expressed in a sufficient amount to produce acetyl-CoA, said acetyl-CoA pathway enzyme comprising an acetyl-CoA synthase, a formate dehydrogenase, a formyltetrahydrofolate synthetase, a methenyltetrahydrofolate cyclohydrolase, a methylenetetrahydrofolate dehydrogenase, and a methylenetetrahydrofolate reductase.

(canceled)

(canceled)

98-100. (canceled)

102-111. (canceled)

(canceled)

118-122. (canceled)

125-131. (canceled)

133-142. (canceled)

(canceled)

149-153. (canceled)

156-172. (canceled)

(canceled)

179-183. (canceled)