Microbial production of pyruvate and pyruvate derivatives
AssigneeUniversity of Georgia Research Foundation, Inc.
InventorsMark A. EITEMAN, Elliot Altman, Yihui Zhu
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Abstract
Microbial production of pyruvate and metabolites derived from pyruvate in cells exhibiting reduced pyruvate dehydrogenase activity compared to wild-type cells. Acetate and glucose are supplied as a carbon sources.
Claims26
A method for making a metabolite comprising pyruvate or a pyruvate derivative, the method comprising:
- providing a bacterial cell exhibiting reduced activity of at least one enzyme in the pyruvate dehydrogenase (PDH) complex of enzymes, compared to a wild-type bacterial cell;
- and
- culturing the bacterial cell in the presence of at least one primary carbon source comprising a compound metabolically upstream of the pyruvate node, and at least one secondary carbon source comprising a compound metabolically downstream from the pyruvate node, to yield the metabolite.
A method for making alanine comprising:
- providing a bacterial cell exhibiting
- (a) reduced activity of at least one enzyme in the pyruvate dehydrogenase (PDH) complex of enzymes, compared to a wild-type bacterial cell, and
- (b) added or increased alanine dehydrogenase activity compared to a wild-type bacterial cell;
- and
- culturing the bacterial cell in the presence of at least one primary carbon source comprising a compound metabolically upstream of the pyruvate node, and at least one secondary carbon source comprising a compound metabolically downstream from the pyruvate node, to yield alanine.
A bacterial cell for use in making a metabolite comprising pyruvate or a pyruvate derivative, the bacterial cell exhibiting reduced activity of at least one enzyme in the pyruvate dehydrogenase (PDH) complex of enzymes, compared to a wild-type bacterial cell;
- wherein the bacterial cell is capable of accumulating the metabolite to a concentration of at least about 3.3 g/L when cultured in the presence of glucose and at least one of acetate and ethanol.
Dependencies are inferred from the claim text.