US 2010/0304448 A1Published application

Method for the enzymatic reduction of alpha- and beta-dehydroamino acids using enoate reductases

OwnerRainer Stürmer, Bernhard Hauer +4inventors

InventorsRainer Stürmer, Bernhard Hauer, Thomas Friedrich, Kurt Faber, Melanie Hall, Clemens Stückler

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Abstract

A method for the enzymatic preparation of amino acids of the general formula (3) or (4) from alpha-dehydroamino acids of the general formula (1) or (2) wherein R1, R2 are independently of one another H, C1-C6 alkyl, C2-C6 alkenyl, an optionally substituted carbo- or heterocyclic, aromatic or nonaromatic radical, or an alkylaryl radical, or a carboxyl radical (—COOR), R3 is H, formyl, acetyl, propionyl, benzyl, benzyloxycarbonyl, BOC, Alloc, R is H, C1-C6 alkyl, aryl, by reducing a compound of the formula (1) or (2) in the presence of a reductase.

Claims10

Dependent claims

A method for the enzymatic preparation of amino acids of the general formula (3) or (4) from alpha-dehydroamino acids of the general formula (1) or (2)

wherein R1, R2 are independently of one another H, C1-C6 alkyl, C2-C6 alkenyl, an optionally substituted carbo- or heterocyclic, aromatic or nonaromatic radical, or an alkylaryl radical, or a carboxyl radical (—COOR),
R3 is H, formyl, acetyl, propionyl, benzyl, benzyloxycarbonyl, BOC, Alloc,
R is H, C1-C6 alkyl, aryl,
by reducing a compound of the formula (1) or (2) in the presence of a reductase
(i) comprising at least one of the polypeptide sequences SEQ ID NO:1, 2, 3, 4, 5, 6, or
(ii) having a functionally equivalent polypeptide sequence which has at least 80% sequence identity with SEQ ID NO:1, 2, 3, 4, 5, 6.

3 more elements

Dependencies are inferred from the claim text.