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US 2010/0304445 A1Published application
Nucleic acid packaging system
OwnerWaclaw Szybalski, Frederick Blattner +2inventors
InventorsWaclaw Szybalski, Frederick Blattner, David Frisch, Douglas Wieczorek
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Abstract
The present invention relates to cloning target nucleic acids using phage packaging mechanisms. Packaging initiation sites may be introduced into the target DNA. Components of a phage packaging system may be combined with the target DNA to package the DNA into phage capsids. The packaged DNA may be used to create a library of target nucleic acids, or it may be sequenced.
Claims12
-76. (canceled)
A method of cloning a nucleic acid from a target nucleic acid comprising:
- (a) contacting a target nucleic acid with a P22HT terminase;
- (b) initiating packaging, whereby the nucleic acid to be cloned from the target nucleic acid is packaged;
- and
- (c) isolating the packaged nucleic acid.
1 more element
Dependencies are inferred from the claim text.