Methods for enhancing the degradation or conversion of cellulosic material
AssigneeNovozymes, Inc.
InventorsASHLEY GARNER, PAUL HARRIS, JASON QUINLAN, RANDALL KRAMER
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Abstract
The present invention relates to methods for degrading or converting a cellulosic material, comprising: treating the cellulosic material with an enzyme composition in the presence of a polypeptide having cellulolytic enhancing activity.
Claims110
A method for degrading or converting a cellulosic material, comprising:
- treating the cellulosic material with an enzyme composition in the presence of a polypeptide having cellulolytic enhancing activity, wherein the polypeptide having cellulolytic enhancing activity is selected from the group consisting of:
- (a) a polypeptide comprising an amino acid sequence having at least 75% sequence identity with the mature polypeptide of SEQ ID NO:
- 2;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under medium-high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 1, (ii) the cDNA sequence contained in the mature polypeptide coding sequence of SEQ ID NO:
- 1, or (iii) the full-length complementary strand of (i) or (ii);
- (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 75% sequence identity with the mature polypeptide coding sequence of SEQ ID NO:
- 1 or the cDNA sequence thereof;
- and
- (d) a variant comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the mature polypeptide of SEQ ID NO:
- 2.
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A method for producing a fermentation product, comprising:
- (A) saccharifying a cellulosic material with an enzyme composition in the presence of a polypeptide having cellulolytic enhancing activity, wherein the polypeptide having cellulolytic enhancing activity is selected from the group consisting of:
- (a) a polypeptide comprising an amino acid sequence having at least 75% sequence identity with the mature polypeptide of SEQ ID NO:
- 2;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under medium-high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 1, (ii) the cDNA sequence contained in the mature polypeptide coding sequence of SEQ ID NO:
- 1, or (iii) the full-length complementary strand of (i) or (ii);
- (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 75% sequence identity with the mature polypeptide coding sequence of SEQ ID NO:
- 1 or the cDNA sequence thereof;
- and
- (d) a variant comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the mature polypeptide of SEQ ID NO:
- 2;
- (B) fermenting the saccharified cellulosic material with one or more (several) fermenting microorganisms;
- and
- (C) recovering the fermentation product from the fermentation.
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A method of fermenting a cellulosic material, comprising:
- fermenting the cellulosic material with one or more (several) fermenting microorganisms, wherein the cellulosic material is saccharified with an enzyme composition in the presence of a polypeptide having cellulolytic enhancing activity, wherein the polypeptide having cellulolytic enhancing activity is selected from the group consisting of:
- (a) a polypeptide comprising an amino acid sequence having at least 75% sequence identity with the mature polypeptide of SEQ ID NO:
- 2;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under medium-high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 1, (ii) the cDNA sequence contained in the mature polypeptide coding sequence of SEQ ID NO:
- 1, or (iii) the full-length complementary strand of (i) or (ii);
- (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 75% sequence identity with the mature polypeptide coding sequence of SEQ ID NO:
- 1 or the cDNA sequence thereof;
- and
- (d) a variant comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the mature polypeptide of SEQ ID NO:
- 2.
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An enzyme composition comprising a polypeptide having cellulolytic enhancing activity and one or more (several) cellulolytic enzymes, wherein the polypeptide having cellulolytic enhancing activity is selected from the group consisting of:
- (a) a polypeptide comprising an amino acid sequence having at least 75% sequence identity with the mature polypeptide of SEQ ID NO:
- 2;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under medium-high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 1, (ii) the cDNA sequence contained in the mature polypeptide coding sequence of SEQ ID NO:
- 1, or (iii) the full-length complementary strand of (i) or (ii);
- (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 75% sequence identity with the mature polypeptide coding sequence of SEQ ID NO:
- 1 or the cDNA sequence thereof;
- and
- (d) a variant comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the mature polypeptide of SEQ ID NO:
- 2.
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Dependencies are inferred from the claim text.