Fluorescent proteins, their production and use
AssigneeEvocatal GmbH
InventorsThorsten Eggert, Sascha Hausmann, Michael Puls
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Abstract
The present invention provides variants of fluorescent proteins, which are improved with regard to their properties for use as reporter proteins and/or in analytics. In particular, variants of fluorescent proteins are provided, which fluoresce brighter, show improved quantum yield and/or have shifted excitation or emission spectra. The fluorescent proteins according to the invention comprise in their LOV domain besides the substitution of a cysteine with an amino acid that does not covalently bind FMN at least one further point mutation.
Claims18
A fluorescent protein having an LOV domain in which at least one cysteine is replaced by another amino acid that does not covalently bind FMN, characterized in that the LOV domain comprises besides the substitution of the at least one cysteine at least one further point mutation for improving the fluorescence intensity, photostability and/or for changing the fluorescence wavelength.
An LOV domain in which at least one cysteine is replaced by another amino acid that does not covalently bind FMN, characterized in that said LOV domain comprises besides the substitution of the at least one cysteine at least one further point mutation for improving the fluorescence intensity, photostability and/or for changing the fluorescence wavelength.
Dependencies are inferred from the claim text.