tRNA SYNTHESIS METHOD, NUCLEIC ACID, AMINOACYL tRNA SYNTHESIS METHOD, AND PROCESS FOR PRODUCTION OF PROTEIN INTRODUCED WITH UNNATURAL AMINO ACID

An aminoacyl-tRNA synthesis method, comprising:

• transcribing in a eukaryotic cell a nucleic acid having a sequence comprising a tRNA nucleotide sequence and a eukaryote-derived tRNA nucleotide sequence linked to a 5′ end of the tRNA nucleotide sequence, in a presence of an aminoacyl-tRNA synthetase corresponding to the tRNA.

A process for producing a protein having an unnatural amino acid introduced therein, the process comprising:

• expressing in a eukaryotic cell an aminoacyl-tRNA synthetase, a nucleic acid having a sequence comprising a tRNA nucleotide sequence and a eukaryote-derived tRNA nucleotide sequence linked to a 5′ end of the tRNA nucleotide sequence that is bindable to an unnatural amino acid in a presence of the aminoacyl-tRNA synthetase, the unnatural amino acid, and a gene of a desired protein having a nonsense mutation at a predetermined position, to integrate the unnatural amino acid at the nonsense mutation position in the protein, thereby expressing a protein having an unnatural amino acid introduced therein.