Methods and apparatus for sorting cells using an optical switch in a microfluidic channel network

A method for cell sorting in a device comprising a microfluidic network having a sample inlet, one or more inlet channels, a main channel, and at least two outlet channels, comprising the steps of:

• receiving cells in a fluidic medium at the sample inlet,
• flowing the cells through the main channel,
• subjecting the cells to a bias flow preferentially resulting in collection of the cells in a first reservoir in fluid communication with a first outlet channel, wherein the bias flow is created by applying asymmetric pressure to one or more of the channels of the microfluidic network,
• identifying a cell to be sorted through application of a lateral force into a second reservoir coupled to a second outlet channel, and
• applying a lateral force on the cell, the lateral force configured to be translated at a non-zero angle down the main channel toward the at least two outlet channels and characterized in that the lateral force is a non-trapping force, whereby the cell is moved transverse to the preferentially biased flow and selectively exits the second outlet channel coupled to the second reservoir.